Desmin Recombinant Rabbit Monoclonal Antibody [SI18-00] (ET1606-30)
Catalog# ET1606-30
Rabbit Monoclonal to Desmin
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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mIHC
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: C2C12 cell lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Rat heart tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 55 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-30) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Desmin Recombinant Rabbit Monoclonal Antibody [SI18-00] (ET1606-30)
Immunogen
Synthetic peptide within human desmin aa 421-470 / 470.
Host
Rabbit
Positive Control
C2C12 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, rat skeletal muscle tissue lysates, C2C12, human endometrium tissue, mouse bladder tissue, mouse colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SI18-00
RRID
APPLICATION DILUTION
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WB
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1:10,000-1:20,000
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IF-Cell
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1:500
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IF-Tissue
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1:200-1:500
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IHC-P
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1:1,000
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FC
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1:1,000
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mIHC
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1:800
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Desmin
SYNONYMS
CMD1I antibody;CSM1 antibody;CSM2 antibody;DES antibody;DESM_HUMAN antibody;Desmin antibody;FLJ12025 antibody;FLJ39719 antibody;FLJ41013 antibody;FLJ41793 antibody;Intermediate filament protein antibody;OTTHUMP00000064865 antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
POST-TRANSLATIONAL MODIFICATION
ADP-ribosylation prevents ability to form intermediate filaments.; Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis.
SUBCELLULAR LOCATION
Cytoplasm, Cell membrane.
FUNCTION
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Desmin makes up attachments between the terminal Z-disc and membrane-associated proteins to form a force-transmitting system. Mutations in the gene encoding for Desmin are associated with adult-onset skeletal myopathy, sporadic disease and mild cardiac involvement.
CITATIONS
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Ma Yunhao,et al
New perspective for Calpain-Mediated regulation of meat Quality: Unveiling the impact on mitochondrial pathway apoptosis in post-mortem
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WB
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Chicken
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Citation
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Qin MC, Li JJ, Zheng YT, et al
Naringin ameliorates liver fibrosis in zebrafish by modulating IDO1-mediated lipid metabolism and inflammatory infiltration
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IF
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Zebrafish
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Citation
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