Positive control:
Lane 1: human skeletal muscle tissue lysate
Lane 2: mouse heart tissue lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
-
Zebrafish
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Desmin Recombinant Rabbit Monoclonal Antibody [SI18-00] (ET1606-30)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Human skeletal muscle tissue lysate, mouse heart tissue lysate, C2C12, human uterus tissue, mouse bladder tissue, mouse skeletal muscle tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SI18-00
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
53 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Desmin
SYNONYMS
CMD1I antibody; CSM1 antibody; CSM2 antibody; DES antibody; DESM_HUMAN antibody; Desmin antibody; FLJ12025 antibody; FLJ39719 antibody; FLJ41013 antibody; FLJ41793 antibody; Intermediate filament protein antibody; OTTHUMP00000064865 antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
POST-TRANSLATIONAL MODIFICATION
ADP-ribosylation prevents ability to form intermediate filaments.; Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis.
SUBCELLULAR LOCATION
Cytoplasm, Cell membrane.
FUNCTION
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Desmin makes up attachments between the terminal Z-disc and membrane-associated proteins to form a force-transmitting system. Mutations in the gene encoding for Desmin are associated with adult-onset skeletal myopathy, sporadic disease and mild cardiac involvement.