Applications
-
WB
-
ICC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Zebrafish
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cytokeratin 13 Recombinant Rabbit Monoclonal Antibody [SN71-09] (ET1611-55)
Immunogen
Synthetic peptide within human cytokeratin 13 aa 111-160 / 458.
Host
Rabbit
Positive Control
Human lung tissue lysates, hybrid fish (crucian-carp) brain tissue lysates, Hela, MCF-7, A549, human tonsil tissue, human breast carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN71-09
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
50 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Cytokeratin 13
SYNONYMS
47 kDa cytokeratin antibody; CK-13 antibody; CK13 antibody; Cytokeratin 13 antibody; Cytokeratin-13 antibody; K13 antibody; K1C13_HUMAN antibody; Ka13 antibody; Keratin 13 antibody; Keratin antibody; keratin type I cytoskeletal 13 antibody; Keratin-13 antibody; Krt-1.13 antibody; Krt1-13 antibody; KRT13 antibody; MGC161462 antibody; MGC3781 antibody; type I cytoskeletal 13 antibody; Type I keratin Ka13 antibody; WSN2 antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
TISSUE SPECIFICITY
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
POST-TRANSLATIONAL MODIFICATION
O-glycosylated; glycans consist of single N-acetylglucosamine residues.
SUBCELLULAR LOCATION
Extracellular exosome, intermediate filament cytoskeleton, keratin filament, nucleus.
FUNCTION
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 13 belongs to the intermediate filament family and is a heterotetramer of two type I acidic and two type II basic keratins. It is generally associated with cytokeratin 4. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder which predominantly affects noncornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa.