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PRODUCT CODE: ET1609-75

Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43] (ET1609-75)

  • Recombinant

Applications

  • WB

  • ICC/IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Cytokeratin 10 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Cytokeratin 10 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of Cytokeratin 10 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-75, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (ET1609-75).<br /> <br /> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (ET1609-75, red) at 1/500 dilution at +4℃ overnight, washed with PBS.<br /> <br /> Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
  • Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (ET1609-75).<br /> <br /> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (ET1609-75, red) at 1/500 dilution at +4℃ overnight, washed with PBS.<br /> <br /> Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
  • Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (ET1609-75) and Vimentin (EM0401).<br /> <br /> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (ET1609-75, red) at 1/400 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS.<br /> <br /> Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) and Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 10 antibody (ET1609-75) at 1/400 dilution.<br /> <br /> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Cytokeratin 10 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC/IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43] (ET1609-75)

Immunogen

Synthetic peptide within human cytokeratin 10 aa 541-584 / 584.

Host

Rabbit

Positive Control

Human skin tissue lysates, Hela, human tonsil tissue, mouse skin tissue, rat skin tissue, human skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST05-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IF

  • 1

  • IHC-P

  • 1:50-1:400

TARGET

UNIPROT #

P13645

PROTEIN NAME

Cytokeratin 10

SYNONYMS

BCIE antibody;BIE antibody;CK 10 antibody;CK-10 antibody;Cytokeratin-10 antibody;EHK antibody;K10 antibody;K1C10_HUMAN antibody;Keratin 10 antibody;Keratin antibody;Keratin type i cytoskeletal 10 antibody;Keratin type I cytoskeletal 59 kDa antibody;Keratin-10 antibody;Keratin10 antibody;KPP antibody;KRT10 antibody;type I cytoskeletal 10 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 10 is a heterotetramer of two type I and two type II keratins, is generally associated with keratin 1, and is seen in all suprabasal cell layers including stratum corneum.