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PRODUCT CODE: ER1802-45

CRMP2 Rabbit Polyclonal Antibody (ER1802-45)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of CRMP2 on different lysates using anti-CRMP2 antibody at 1/5,000 dilution.<br /> Positive control:<br /> Lane 1: Mouse brain tissue Lane 2: SH-SY-5Y
  • Western blot analysis of CRMP2 on different lysates using anti-CRMP2 antibody at 1/5,000 dilution.<br /> Positive control:<br /> Lane 1: Mouse brain tissue Lane 2: SH-SY-5Y
  • ICC staining CRMP2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CRMP2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CRMP2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CRMP2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CRMP2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CRMP2 antibody. Counter stained with hematoxylin.
Western blot analysis of CRMP2 on different lysates using anti-CRMP2 antibody at 1/5,000 dilution.
Positive control:
Lane 1: Mouse brain tissue Lane 2: SH-SY-5Y

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CRMP2 Rabbit Polyclonal Antibody (ER1802-45)

Immunogen

Recombinant protein within human crmp2 aa 90-320.

Host

Rabbit

Positive Control

Mouse brain tissue lysate, SH-SY-5Y, A549, SiHa, human tonsil tissue, human colon tissue, human breast cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

Q16555

PROTEIN NAME

CRMP2

SYNONYMS

Collapsin response mediator protein 2 antibody; Collapsin response mediator protein antibody; Collapsin response mediator protein hCRMP 2 antibody; CRAM antibody; CRMP 2 antibody; CRMP-2 antibody; CRMP2 antibody; DHPRP 2 antibody; DHPRP2 antibody; Dihydropyrimidinase 2 antibody; Dihydropyrimidinase like 2 antibody; Dihydropyrimidinase like 2 long form antibody; Dihydropyrimidinase related protein 2 antibody; Dihydropyrimidinase-related protein 2 antibody; DPYL 2 antibody; DPYL2 antibody; DPYL2_HUMAN antibody; DPYSL 2 antibody; Dpysl2 antibody; DRP-2 antibody; DRP2 antibody; Musunc 33 antibody; Musunc33 antibody; N2A3 antibody; TOAD 64 antibody; TOAD64 antibody; ULIP 2 protein antibody; ULIP-2 antibody; Ulip2 antibody; Unc-33-like phosphoprotein 2 antibody

SEQUENCE SIMILARITIES

Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.; Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration (By similarity). Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B.

SUBCELLULAR LOCATION

Cytosol. Cytoskeleton. Membrane.

FUNCTION

Collapsin response mediator proteins (CRMPs), including CRMP-1 (DRP-1), CRMP-2 (DRP-2 or TOAD64), CRMP-3 (DRP-4), CRMP-4 (DRP-3) and CRMP-5 (DRP-5), mediate signal transduction after exposure of neural cells to the axon guidance molecule Semaphorin 3A (SEMA3A)/collapsin. CRMPs are present in the developing cerebral cortex and neocortical neurons and are responsive to SEMA3A. In the adult brain, the expression of CRMPs is dramatically downregulated. However, they remain expressed in structures that retain their capacity for differentiation and plasticity. CRMP-2 is involved in axonal growth and guidance. The human CRMP-2 gene maps to 8p21.2, a chromosomal region that has been previously shown to have a significant linkage to schizophrenia and to several deficit symptoms of schizophrenia.