PRODUCT CODE: ET7107-27

CRM1 Recombinant Rabbit Monoclonal Antibody [JB35-22] (ET7107-27)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of CRM1 on 293T and Hela cell lysates using anti-CRM1 antibody at 1/500 dilution.
  • Western blot analysis of CRM1 on 293T and Hela cell lysates using anti-CRM1 antibody at 1/500 dilution.
  • ICC staining CRM1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CRM1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CRM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with CRM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of CRM1 on 293T and Hela cell lysates using anti-CRM1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CRM1 Recombinant Rabbit Monoclonal Antibody [JB35-22] (ET7107-27)

Immunogen

Recombinant protein within c terminal human crm1.

Host

Rabbit

Positive Control

293T, Hela, A431, LOVO, SiHa, K562, rat skeletal muscle tissue, human lung cancer tissue, human tonsil tissue, mouse skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB35-22

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

123 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CRM1

SYNONYMS

Chromosome region maintenance 1 protein homolog antibody; CRM 1 antibody; CRM1 homolog antibody; DKFZp686B1823 antibody; emb antibody; Exp 1 antibody; Exp1 antibody; Exportin 1 antibody; Exportin-1 antibody; Exportin1 antibody; XPO 1 antibody; xpo1 antibody; XPO1_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the exportin family.

TISSUE SPECIFICITY

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Protein transport across the nucleus is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin alpha1 and karyopherin beta1 subunits. p62 glycoprotein is a nucleoporin that is not only involved in the nuclear import of proteins, but also the export of nascent mRNA strands. NTF2 (nuclear transport factor 2) interacts with nucleoporin p62 as a homodimer composed of two monomers, and may be an obligate component of functional p62. CRM1 has been shown to be an export receptor for leucine-rich proteins that contain the nuclear export signal (NES).