Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Connexin-26 Rabbit Polyclonal Antibody (ER1902-42)
Immunogen
Synthetic peptide within rat connexin-26 aa 90-130.
Host
Rabbit
Positive Control
Human skin tissue lysates, rat testis tissue, human liver tissue, mouse colon tissue, A431.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
26 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB:1:500-1:1,000
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Connexin-26
SYNONYMS
connexin 26 antibody; Connexin-26 antibody; Cx26 antibody; CXB2_HUMAN antibody; DFNA3 antibody; DFNB1 antibody; Gap junction beta-2 protein antibody; GJB2 antibody; HID antibody; KID antibody; NSRD1 antibody; PPK antibody
SEQUENCE SIMILARITIES
Belongs to the connexin family. Beta-type (group I) subfamily.
SUBCELLULAR LOCATION
Cell membrane, gap junction.
FUNCTION
Gap junction beta-2 protein (GJB2), also known as connexin 26 (Cx26) — is a protein that in humans is encoded by the GJB2 gene. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into two categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 (GJA1) is designated alpha-1 gap junction protein, whereas GJB1 (CX32), and GJB2 (CX26; this protein) are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that GJB1 and GJB2 are more homologous to each other than either of them is to gap junction protein, alpha GJA1. Connexin 26 also plays a role in tumor suppression through mediation of the cell cycle. The abnormal expression of Cx26, correlated with several types of human cancers, may serve as a prognostic factor for cancers such as colorectal cancer, breast cancer, and bladder cancer. Furthermore, Cx26 over-expression is suggested to promote cancer development by facilitating cell migration and invasion and by stimulating the self-perpetuation ability of cancer stem cells.