Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314] (ET1608-10)
Catalog# ET1608-10
Rabbit Monoclonal to Cleaved PARP
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WB
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IF-Cell
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IP
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Human
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 1 minute 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-10) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Safety datasheet
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- MSDS_HUABIO.pdf
- MSDS_HUABIO.pdf
- MSDS_ET1608-10_Europe.pdf
- No MSDS Found
COA DOWNLOAD
- No COA Found
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314] (ET1608-10)
Immunogen
Synthetic peptide within human parp1 aa 200-249 / 1,014.
Host
Rabbit
Positive Control
Jurkat cell lysate, A549 cell lysate, Hela, Daudi cell lysates.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU0314
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100-1:250
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 89 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cleaved PARP
SYNONYMS
ADP-ribosyltransferase diphtheria toxin-like 1 antibody;ADPRT 1 antibody;ADPRT antibody;ADPRT1 antibody;APOPAIN antibody;ARTD1 antibody;NAD(+) ADP-ribosyltransferase 1 antibody;PARP antibody;PARP-1 antibody;PARP1 antibody;PARP1_HUMAN antibody;Poly [ADP-ribose] polymerase 1 antibody;Poly ADP ribose polymerase 1 antibody;Poly[ADP-ribose] synthase 1 antibody;PPOL antibody;SCA1 antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes such as DNA repair, genomic stability, and programmed cell death. The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.
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