Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314] (ET1608-10)
Catalog# ET1608-10
Rabbit Monoclonal to Cleaved PARP
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WB
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IF-Cell
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IP
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FC
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Human
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: A549 cell lysate
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314] (ET1608-10)
Immunogen
Synthetic peptide within human parp1 aa 200-249 / 1,014.
Host
Rabbit
Positive Control
Jurkat cell lysate, A549 cell lysate, Hela, Daudi cell lysates.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU0314
APPLICATION DILUTION
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WB
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1:500
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FC
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1:50-1:100
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IF-Cell
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 89 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cleaved PARP
SYNONYMS
ADP-ribosyltransferase diphtheria toxin-like 1 antibody;ADPRT 1 antibody;ADPRT antibody;ADPRT1 antibody;APOPAIN antibody;ARTD1 antibody;NAD(+) ADP-ribosyltransferase 1 antibody;PARP antibody;PARP-1 antibody;PARP1 antibody;PARP1_HUMAN antibody;Poly [ADP-ribose] polymerase 1 antibody;Poly ADP ribose polymerase 1 antibody;Poly[ADP-ribose] synthase 1 antibody;PPOL antibody;SCA1 antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes such as DNA repair, genomic stability, and programmed cell death. The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.
CITATIONS
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Chen, Y., Lu, Z., Qi, C., Yu, C., Li, Y., Huan, W., Wang, R., Luo, W., Shen, D., Ding, L., Ren, L., Xie, H., Xue, D., Wang, M., Ni, K., Xia, L., Qian, J., & Li, G.
N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma
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WB
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Human
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Citation
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Chen, X. B., Zhu, H. Y., Bao, K., Jiang, L., Zhu, H., Ying, M. D., He, Q. J., Yang, B., Sheng, R., & Cao, J.
Bis-isatin derivatives: design, synthesis, and biological activity evaluation as potent dimeric DJ-1 inhibitors. Acta pharmacologica Sinica, 10.1038/s41401-020-00600-5. Advance online publication.
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Liu, Xia et al.
Silencing RRM2 inhibits multiple myeloma by targeting the Wnt/β-catenin signaling pathway. | Molecular Medicine Reports [2019]
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WB
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multiple myeloma cell
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Citation
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Weng, Q., Sun, H., Fang, C., Xia, F., Liao, H., Lee, J., Wang, J., Xie, A., Ren, J., Guo, X., Li, F., Yang, B., & Ling, D.
Catalytic activity tunable ceria nanoparticles prevent chemotherapy-induced acute kidney injury without interference with chemotherapeutics. Nature communications, 12(1), 1436.
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WB
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Human
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Citation
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Shao, C., Yu, Z., Luo, T., Zhou, B., Song, Q., Li, Z., Yu, X., Jiang, S., Zhou, Y., Dong, W., Zhou, X., Wang, X., & Song, H.
Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro
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WB
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Monkey
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Citation
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-
Hu, J., Cao, J., Jin, R., Zhang, B., Topatana, W., Juengpanich, S., Li, S., Chen, T., Lu, Z., Cai, X., & Chen, M.
Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth
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WB
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Human
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Citation
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-
Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway
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WB
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Human
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Citation
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-
Reduced HMGB1 expression contributed to lapatinib-induced cutaneous injury
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WB
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Human
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Citation
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Long, X., Chen, L., Yang, J., Dong, T., Cheng, Q., Wang, W., Zou, Y., Su, Y., Dai, W., Chen, B., & Zhou, X.
Network-based pharmacology and in vitro validation reveal that galangin induces apoptosis in bladder cancer cells by promoting the p53 signaling pathway
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WB
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Human
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Citation
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Liu, X., Cui, S., Dan, C., Li, W., Xie, H., Li, C., & Shi, L.
Phellinus baumii Polyphenol: A Potential Therapeutic Candidate against Lung Cancer Cells
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WB
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Human
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Citation
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