Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314] (ET1608-10)

UNIPROT #

P09874

PROTEIN NAME

Cleaved PARP

SYNONYMS

ADP-ribosyltransferase diphtheria toxin-like 1 antibody;ADPRT 1 antibody;ADPRT antibody;ADPRT1 antibody;APOPAIN antibody;ARTD1 antibody;NAD(+) ADP-ribosyltransferase 1 antibody;PARP antibody;PARP-1 antibody;PARP1 antibody;PARP1_HUMAN antibody;Poly [ADP-ribose] polymerase 1 antibody;Poly ADP ribose polymerase 1 antibody;Poly[ADP-ribose] synthase 1 antibody;PPOL antibody;SCA1 antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes such as DNA repair, genomic stability, and programmed cell death. The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.