PRODUCT CODE: ET1608-10

Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314] (ET1608-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of Cleaved PARP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Jurkat cell lysate<br />
Lane 2: A549 cell lysate
  • Western blot analysis of Cleaved PARP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Jurkat cell lysate<br />
Lane 2: A549 cell lysate
  • Flow cytometric analysis of Cleaved PARP was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-10, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Cleaved PARP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: A549 cell lysate

Applications

  • WB

  • ICC

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314] (ET1608-10)

Immunogen

Synthetic peptide within human parp1 aa 200-249 / 1,014.

Host

Rabbit

Positive Control

Jurkat cell lysate, A549 cell lysate, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU0314

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

89 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cleaved PARP

SYNONYMS

ADP-ribosyltransferase diphtheria toxin-like 1 antibody; ADPRT 1 antibody; ADPRT antibody; ADPRT1 antibody; APOPAIN antibody; ARTD1 antibody; NAD(+) ADP-ribosyltransferase 1 antibody; PARP antibody; PARP-1 antibody; PARP1 antibody; PARP1_HUMAN antibody; Poly [ADP-ribose] polymerase 1 antibody; Poly ADP ribose polymerase 1 antibody; Poly[ADP-ribose] synthase 1 antibody; PPOL antibody; SCA1 antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity. This antibody recognizes the apoptosis-specific 89 kDa catalytic domain fragment, but it does not recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment.

CITATIONS

  • Liu, Xia et al.

    Silencing RRM2 inhibits multiple myeloma by targeting the Wnt/β-catenin signaling pathway. | Molecular Medicine Reports [2019]

  • Chen, X. B., Zhu, H. Y., Ba...

    Bis-isatin derivatives: design, synthesis, and biological activity evaluation as potent dimeric DJ-1 inhibitors. Acta pharmacologica Sinica, 10.1038/s41401-020-00600-5. Advance online publication.

  • Weng, Q., Sun, H., Fang, C....

    Catalytic activity tunable ceria nanoparticles prevent chemotherapy-induced acute kidney injury without interference with chemotherapeutics. Nature communications, 12(1), 1436.