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PRODUCT CODE: M1412-2

CLDN7 Mouse Monoclonal Antibody [6-G3] (M1412-2)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis on SW480 cell lysates using anti- CLDN7 mouse mAb.
  • Western blot analysis on SW480 cell lysates using anti- CLDN7 mouse mAb.
  • ICC staining CLDN7 in SW480 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded Human small intestine using anti-CLDN7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1412-2, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded Human kidney using anti-CLDN7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1412-2, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis on SW480 cell lysates using anti- CLDN7 mouse mAb.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CLDN7 Mouse Monoclonal Antibody [6-G3] (M1412-2)

Immunogen

Peptide

Host

Mouse

Positive Control

SW480

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

6-G3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

~22kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:100-1:200

  • IHC-P:1:100-1

  • 500

TARGET

UNIPROT #

O95471

PROTEIN NAME

CLDN7

SYNONYMS

CEPTR L2 antibody; CEPTRL 2 antibody; CEPTRL2 antibody; Claudin 1 antibody; Claudin 9 antibody; Claudin-7 antibody; Claudin1 antibody; Claudin7 antibody; Claudin9 antibody; CLD7_HUMAN antibody; CLDN 7 antibody; CLDN-7 antibody; CLDN7 antibody; Clostridium perfringens enterotoxin receptor like 2 antibody; CPETR L2 antibody; CPETRL 2 antibody; CPETRL2 antibody; Hs.84359 antibody

SEQUENCE SIMILARITIES

Belongs to the claudin family.

TISSUE SPECIFICITY

Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. It belongs to the group of claudins.Claudins, such as CLDN7, are involved in the formation of tight junctions between epithelial cells. Tight junctions restrict lateral diffusion of lipids and membrane proteins, and thereby physically define the border between the apical and basolateral compartments of epithelial cells. It is expressed in kidney, lung and prostate. Claudin-7 is down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.