PRODUCT CODE: ET1606-31

CD8 alpha Recombinant Rabbit Monoclonal Antibody [SI18-01] (ET1606-31)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

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+
Western blot analysis of CD8 alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: LOVO cell lysate
  • Western blot analysis of CD8 alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: LOVO cell lysate
  • ICC staining of CD8 alpha in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-31, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of CD8 alpha in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-31, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CD8 alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: LOVO cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CD8 alpha Recombinant Rabbit Monoclonal Antibody [SI18-01] (ET1606-31)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, LOVO cell lysate, Hela, CRC, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SI18-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD8 alpha

SYNONYMS

alpha polypeptide (p32) antibody; CD8 antibody; CD8 antigen alpha polypeptide antibody; CD8 antigen alpha polypeptide (p32) antibody; CD8a antibody; CD8A antigen antibody; CD8A molecule antibody; CD8A_HUMAN antibody; Leu2 antibody; Leu2 T lymphocyte antigen antibody; Ly3 antibody; LYT3 antibody; MAL antibody; OKT8 T cell antigen antibody; OTTHUMP00000160760 antibody; OTTHUMP00000160764 antibody; OTTHUMP00000203528 antibody; OTTHUMP00000203721 antibody; p32 antibody; T cell antigen Leu2 antibody; T cell co receptor antibody; T-cell surface glycoprotein CD8 alpha chain antibody; T-lymphocyte differentiation antigen T8/Leu-2 antibody; T8 T cell antigen antibody; T8/Leu-2 T-lymphocyte differentiation antigen antibody

TISSUE SPECIFICITY

CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. Less frequently, CD8 can be expressed as a CD8A homodimer. A subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes and dendritic cells expresses CD8A homodimers. Expressed at the cell surface of plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.

POST-TRANSLATIONAL MODIFICATION

Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts.; O-glycosylated.; Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.

SUBCELLULAR LOCATION

Cell membrane, Secreted.

FUNCTION

The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 (CD8-α and CD8-β) co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8 proteins; T helper cells express CD4 proteins and T cytotoxic cells display CD8 proteins. CD8s are cell surface glycoproteins that exist as two chain complex (αα or αβ) receptors that bind class I MHC molecules presented by the antigen-presenting cell (APC). A primary function of CD8 proteins is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. An additional role for CD8-expressing T cells may be to maintain low levels of HIV expression.

CITATIONS

  • Li, Yi-Ming et al.

    Programmed Death Ligand 1 Indicates Pre-Existing Adaptive Immune Response by Tumor-Infiltrating CD8+ T Cells in Non-Small Cell Lung Cancer. | International Journal of Molecular Sciences [2019]