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PRODUCT CODE: ER1902-73

CD1a Rabbit Polyclonal Antibody (ER1902-73)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of CD1a on rat skin tissue lysate lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-73, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD1a on rat skin tissue lysate lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-73, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of CD1a on human skin tissue lysate lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of CD1a was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-73, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of CD1a on rat skin tissue lysate lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-73, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CD1a Rabbit Polyclonal Antibody (ER1902-73)

Immunogen

Recombinant protein within human cd1a aa 1-152 / 327.

Host

Rabbit

Positive Control

Rat skin tissue lysates, 293.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

ProA affinity purified.

MOLECULAR WEIGHT

Predicted band size 37 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P:1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P06126

PROTEIN NAME

CD1a

SYNONYMS

CD 1a antibody; CD1 antibody; CD1a antibody; CD1A Antigen antibody; CD1A antigen, a polypeptide antibody; CD1a molecule antibody; CD1A_HUMAN antibody; cluster of differentiation 1 A antibody; cortical thymocyte antigen CD1A antibody; differentiation antigen CD1 alpha 3 antibody; epidermal dendritic cell marker CD1a antibody; FCB 6 antibody; FCB6 antibody; HTA 1 antibody; HTA1 antibody; hTa1 thymocyte antigen antibody; OTTHUMP00000018907 antibody; R 4 antibody; R4 antibody; T 6 antibody; T-cell surface antigen T6/Leu-6 antibody; T-cell surface glycoprotein CD1a antibody; T6 antibody

TISSUE SPECIFICITY

Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.

SUBCELLULAR LOCATION

Cell membrane. Endosome membrane.

FUNCTION

The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. CD1 antigens are associated with β-2-Microglobulin and expressed on cortical thymocytes, Langerhans cells, a B cell subset and some dendritic cells. Specifically, CD1A is a marker for Langerhans cell histiocytosis (LCH) and is found on interdigitating cells. Adaptor-protein complexes and CD1-associated chaperones control CD1 trafficking, and the development and activation of CD1-restricted T cells. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells. The human CD1 genes are all closely linked in a cluster mapping at chromosome 1q23.1.