CD14 Recombinant Rabbit Monoclonal Antibody [SC69-02] (ET1610-85)
Catalog# ET1610-85
Rabbit Monoclonal to CD14
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WB
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IF-Cell
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IHC-P
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mIHC
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FC
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Human
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Mouse
Lane 1: THP-1 cell lysate
Lane 2: RAW264.7 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 60 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-85) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD14 Recombinant Rabbit Monoclonal Antibody [SC69-02] (ET1610-85)
Immunogen
Synthetic peptide within human cd14 aa 310-335 / 375.
Host
Rabbit
Positive Control
THP-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysates, A549, NCCIT, NIH/3T3, LO2, human tonsil tissue, human liver tissue, human colon carcinoma tissue, human spleen tissue, human uterus tissue, human lymph nodes tissue, human cervical cancer.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC69-02
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:200-1:800
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mIHC
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1:800-1:1,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 40 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD14
SYNONYMS
CD 14 antibody;CD_antigen=CD14 antibody;CD14 antibody;CD14 antigen antibody;CD14 molecule antibody;CD14_HUMAN antibody;LPS-R antibody;Mo2 antibody;Monocyte differentiation antigen CD14 antibody;Monocyte differentiation antigen CD14 urinary form antibody;Monocyte differentiation antigen CD14, membrane-bound form antibody;Myeloid cell specific leucine rich glycoprotein antibody;Myeloid cell-specific leucine-rich glycoprotein antibody
TISSUE SPECIFICITY
Detected on macrophages (at protein level). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
POST-TRANSLATIONAL MODIFICATION
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
SUBCELLULAR LOCATION
Cell membrane, Secreted, Golgi apparatus, Membrane raft.
FUNCTION
Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.
CITATIONS
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Wang, M., Li, Y., Yang, J., Wang, X., & Zhang, L.
Genes related to allergen exposure in allergic rhinitis: a gene-chip-based study in a mouse model
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IHC-P
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Mouse
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Citation
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Nrf2 deficiency attenuates atherosclerosis by reducing LOX-1-mediated proliferation and migration of vascular smooth muscle cells
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WB
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IF
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Mouse
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Citation
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