PRODUCT CODE: EM1701-43

CD130 Mouse Monoclonal Antibody [B11-B9-A8] (EM1701-43)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

Western blot analysis of CD130 on CD130 recombinant protein using anti-CD130 antibody at 1/5,000 dilution.
  • Western blot analysis of CD130 on CD130 recombinant protein using anti-CD130 antibody at 1/5,000 dilution.
  • Western blot analysis of CD130 on Raji cell (1) and mouse lung tissue (2) lysates using anti-CD130 antibody at 1/500 dilution.
  • ICC staining CD130 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CD130 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD130 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD130 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD130 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD130 antibody. Counter stained with hematoxylin.
Western blot analysis of CD130 on CD130 recombinant protein using anti-CD130 antibody at 1/5,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CD130 Mouse Monoclonal Antibody [B11-B9-A8] (EM1701-43)

Immunogen

Recombinant protein within human cd130 238-422 / 918.

Host

Mouse

Positive Control

CD130 recombinant protein, Raji cell and mouse lung tissue lysates, HUVEC, LOVO, human prostate tissue, human liver tissue, human kidney tissue, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

B11-B9-A8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

103 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CD130

SYNONYMS

CD130 antibody; CD130 antigen antibody; CDw130 antibody; gp130 antibody; GP130 RAPS antibody; IL 6R beta antibody; IL-6 receptor subunit beta antibody; IL-6R subunit beta antibody; IL-6R-beta antibody; IL-6RB antibody; IL6 ST antibody; IL6RB_HUMAN antibody; IL6ST antibody; Interleukin 6 receptor subunit beta antibody; Interleukin receptor beta chain antibody; Interleukin-6 receptor subunit beta antibody; Interleukin-6 signal transducer antibody; Membrane glycoprotein 130 antibody; Membrane glycoprotein gp130 antibody; Oncostatin M receptor antibody; Oncostatin M receptor alpha subunit antibody; Oncostatin-M receptor subunit alpha antibody

SEQUENCE SIMILARITIES

Belongs to the type I cytokine receptor family. Type 2 subfamily.

TISSUE SPECIFICITY

Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. Expressed in blood serum (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Ser-782 down-regulates cell surface expression.; Heavily N-glycosylated. Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism. Has a role in embryonic development. Does not bind IL6. Essential for survival of motor and sensory neurons and for differentiation of astrocytes. Required for expression of TRPA1 in nociceptive neurons. Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation. Required for normal trabecular bone mass and cortical bone composition.