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Casein Kinase 1 alpha Recombinant Rabbit Monoclonal Antibody [PSH04-46]

Usd: 385

Specification

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Catalog# HA722131

Casein Kinase 1 alpha Recombinant Rabbit Monoclonal Antibody [PSH04-46]

Application

  • WB

  • IF-Cell

  • FC

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Casein Kinase 1 alpha Recombinant Rabbit Monoclonal Antibody [PSH04-46]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CSNK1A1 aa 1-337 / 337.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC, IP

Molecular Weight

Predicted band size: 39 kDa

Positive Control

HCT 116 cell lysate, HeLa cell lysate, HEK-293 cell lysate, MCF7 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HCT 116.

Conjugation

unconjugated

Clone Number

PSH04-46

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:500

  • FC

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2 (By similarity). May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (By similarity).

Background References

1. Ma Z et al. Knockdown of Csnk1a1 results in preimplantation developmental arrest in mice. Theriogenology. 2023 Mar

2. Pan M et al. CSNK1A1/CK1α suppresses autoimmunity by restraining the CGAS-STING1 signaling. Autophagy. 2024 Feb

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome, centromere, kinetochore, Nucleus speckle, cilium basal body, spindle.

UNIPROT

SwissProt: P48729 Human

SwissProt: Q8BK63 Mouse

SwissProt: P97633 Rat

Synonyms

Casein kinase 1 alpha 1 antibody

Casein kinase I isoform alpha antibody

CK1 antibody

CK1A antibody

CKI alpha antibody

CKI-alpha antibody

CKIa antibody

Clock regulator kinase antibody

Csnk1a1 antibody

Down regulated in lung cancer antibody

Expand

Images

  • Western blot analysis of Casein Kinase 1 alpha on different lysates with Rabbit anti-Casein Kinase 1 alpha antibody (<a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: HEK-293 cell lysate (20 µg/Lane)<br />Lane 4: MCF7 cell lysate (20 µg/Lane)<br />Lane 5: Jurkat cell lysate (20 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 7: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 8: PC-12 cell lysate (20 µg/Lane)<br />Lane 9: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 10: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 39 kDa<br />Observed band size: 34 kDa<br /><br />Exposure time: 15 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Casein Kinase 1 alpha on different lysates with Rabbit anti-Casein Kinase 1 alpha antibody (HA722131) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: HCT 116 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: HEK-293 cell lysate (20 µg/Lane)
    Lane 4: MCF7 cell lysate (20 µg/Lane)
    Lane 5: Jurkat cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 7: RAW264.7 cell lysate (20 µg/Lane)
    Lane 8: PC-12 cell lysate (20 µg/Lane)
    Lane 9: Mouse brain tissue lysate (40 µg/Lane)
    Lane 10: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 39 kDa
    Observed band size: 34 kDa

    Exposure time: 15 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722131) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HCT 116 cells labeling Casein Kinase 1 alpha with Rabbit anti-Casein Kinase 1 alpha antibody (<a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a>) at 1/500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 1 alpha antibody (<a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HCT 116 cells labeling Casein Kinase 1 alpha with Rabbit anti-Casein Kinase 1 alpha antibody (HA722131) at 1/500 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 1 alpha antibody (HA722131) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of HCT 116 cells labeling Casein Kinase 1 alpha.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HCT 116 cells labeling Casein Kinase 1 alpha.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722131, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (<a href="/products/NBI01H" style="font-weight: bold;text-decoration: underline;">NBI01H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722131" style="font-weight: bold;text-decoration: underline;">HA722131</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute 11 seconds; ECL: K1801

    Casein Kinase 1 alpha was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722131 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722131 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA722131 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA722131 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute 11 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Casein Kinase 1 alpha Recombinant Rabbit Monoclonal Antibody [PSH04-46] - BSA and Azide free

Application: WB,IF-Cell,FC,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated