Carbonic anhydrase 2 Rabbit Polyclonal Antibody
Catalog# ER1802-72
Carbonic anhydrase 2 Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
Carbonic anhydrase 2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Carbonic anhydrase 2 aa 40-240.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 29 kDa
Positive Control
293 cell lysate, THP-1 cell lysate, HL-60 cell lysate, 293T, Hela, LOVO, human liver tissue, human colon cancer tissue, human appendix tissue, human stomach cancer tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
Target
Function
Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. Carbonic anhydrase II (gene name CA2), is one of sixteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of bicarbonate ions in the proximal tubule. Loss of carbonic anhydrase activity in bones impairs the ability of osteoclasts to promote bone resorption, leading to osteopetrosis.
Background References
1. Xuan GS. et. al. Inhibition of carbonic anhydrase II by sulfonamide derivatives. Pharmazie. 2021 Sep
2. Annan DA. et. al. Carbonic anhydrase 2 (CAII) supports tumor blood endothelial cell survival under lactic acidosis in the tumor microenvironment. Cell Commun Signal. 2019 Dec
Sequence Similarity
Belongs to the alpha-carbonic anhydrase family.
Subcellular Location
Cytoplasm. Cell membrane.
UNIPROT
Synonyms
can antibody
CAN_ECOLI antibody
Carbonate dehydratase 2 antibody
Carbonic anhydrase 2 antibody
Images
-
Western blot analysis of Carbonic anhydrase 2 on different lysates with Rabbit anti-Carbonic anhydrase 2 antibody (ER1802-72) at 1/500 dilution.
Lane 1: 293 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: HL-60 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
ICC staining Carbonic anhydrase 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining Carbonic anhydrase 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining Carbonic anhydrase 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
