Positive control:
Lane 1: THP-1 cell lysate, untreated
Lane 2: HL-60 cell lysate, untreated
Applications
-
WB
-
ICC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Carbonic anhydrase 2 Mouse Monoclonal Antibody [11A1] (EM1801-08)
Immunogen
Recombinant protein within human carbonic anhydrase 2 aa 63-240 / 260.
Host
Mouse
Positive Control
THP-1, HL-60, rat liver tissue, human colon tissue, human kidney tissue, human stomach cancer tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
11A1
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
29 kDa
Isotype
IgG2a
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
ICC:1:50-1:200
-
IHC-P:1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Carbonic anhydrase 2
SYNONYMS
CA 2 antibody; CA II antibody; CA-II antibody; Ca2 antibody; CAC antibody; CAH2_HUMAN antibody; CAII antibody; Car 2 antibody; Car2 antibody; Carbonate dehydratase II antibody; Carbonic anhydrase 2 antibody; Carbonic anhydrase B antibody; Carbonic anhydrase C antibody; Carbonic anhydrase C, formerly antibody; Carbonic anhydrase II antibody; Carbonic dehydratase antibody; epididymis luminal protein 76 antibody; Epididymis secretory protein Li 282 antibody; HEL-76 antibody; HEL-S-282 antibody
SEQUENCE SIMILARITIES
Belongs to the alpha-carbonic anhydrase family.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression.