PRODUCT CODE: 0912-2

C-myc Antibody (0912-2)

Applications

  • WB

REACTIVITY

Western blot analysis on myc-tagged recombinant protein with (A) and A549 cell lysates (B).
  • Western blot analysis on myc-tagged recombinant protein with (A) and A549 cell lysates (B).
Western blot analysis on myc-tagged recombinant protein with (A) and A549 cell lysates (B).

Applications

  • WB

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

C-myc Antibody (0912-2)

Immunogen

Peptide

Host

Rabbit

Positive Control

Recombinant protein, A549

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

57 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

Myc proto-oncogene protein

GENE NAME

MYC

SYNONYMS

bHLHe39

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.; Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).

SUBCELLULAR LOCATION

Nucleus, nucleoplasm. Nucleus, nucleolus.

FUNCTION

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).

CITATIONS

  • Zan, Jie et al.

    Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6. | Frontiers in Cellular and Infection Microbiology [2017]

  • Fan, Xiaojing et al.

    The ColRS-Regulated Membrane Protein Gene XAC1347 Is Involved in Copper Homeostasis and hrp Gene Expression in Xanthomonas citri subsp. citri. | Frontiers in Microbiology [2018]

  • Huang, Chao et al.

    MORF2 tightly associates with MORF9 to regulate chloroplast RNA editing in Arabidopsis. | Plant Science : An International Journal of Experimental Plant Biology [2019]