PRODUCT CODE: M1305-2

Beta tubulin Mouse Monoclonal Antibody [A1-A4] (M1305-2)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis on 1、NIH/3T3 2、Hela lysates using anti-β-tubulin Mouse mAb (Cat. # M1305-2).
  • Western blot analysis on 1、NIH/3T3 2、Hela lysates using anti-β-tubulin Mouse mAb (Cat. # M1305-2).
  • Immunofluorescent staining on SKOV-3 cells using anti-β-tubulin mouse mAb.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SHSY-5Y cells with beta tubulin antibody at 1/50 dilution (black) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis on 1、NIH/3T3 2、Hela lysates using anti-β-tubulin Mouse mAb (Cat. # M1305-2).

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Beta tubulin Mouse Monoclonal Antibody [A1-A4] (M1305-2)

Immunogen

Synthetic peptide.

Host

Mouse

Positive Control

NIH/3T3, Hela, SKOV-3, human tonsil tissue, human spleen tissue, human colon tissue, rat brain tissue, SHSY-5Y cells.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A1-A4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG3

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • ICC

  • 1:200-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Beta tubulin

SYNONYMS

beta 3 tubulin antibody; beta-4 antibody; CDCBM antibody; CDCBM1 antibody; CFEOM3 antibody; CFEOM3A antibody; FEOM3 antibody; M(beta)3 antibody; M(beta)6 antibody; MC1R antibody; Neuron specific beta III Tubulin antibody; Neuron-specific class III beta-tubulin antibody; QccE-11995 antibody; QccE-15186 antibody; TBB3_HUMAN antibody; Tubb 3 antibody; TUBB3 antibody; TUBB4 antibody; Tubulin beta 3 antibody; Tubulin beta 3 chain antibody; Tubulin beta 4 antibody; Tubulin beta III antibody; Tubulin beta-3 chain antibody; Tubulin beta-4 chain antibody; Tubulin beta-III antibody

SEQUENCE SIMILARITIES

Belongs to the tubulin family.

TISSUE SPECIFICITY

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

POST-TRANSLATIONAL MODIFICATION

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Tubulins is one of several members of a small family of globular proteins. The most common members of the tubulins family are α-tubulins and β-tubulins. The beta-tubulins (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulins heterodimer, it is coded by multiple tubulins genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulins may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.

CITATIONS

  • Wang, Q et al.

    JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells. | Oncogenesis [2017]

  • Guo, Na et al.

    Alternative transcription start site selection in Mr-OPY2 controls lifestyle transitions in the fungus Metarhizium robertsii. | Nature Communications [2017]

  • Yu, Tingting et al.

    P2Y12 regulates microglia activation and excitatory synaptic transmission in spinal lamina II neurons during neuropathic pain in rodents. | Cell Death & Disease [2019]

  • Zhang, Xueyun et al.

    Glucose-6-Phosphate Upregulates Txnip Expression by Interacting With MondoA. | Frontiers in Molecular Biosciences [2019]