Positive control:
Lane 1: NCCIT
Lane 2: NIH/3T3
Lane 3: PC12
Lane 4: Mouse heart
Lane 5: F9
Lane 6: zebrafish brain
Lane 7: Hela
Applications
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WB
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ICC
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IHC-P
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FC
REACTIVITY
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Human
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Mouse
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Rat
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Zebrafish
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
beta-tubulin Mouse Monoclonal Antibody [1-B11] (EM0103)
Immunogen
Synthetic peptide within human beta tubulin aa 51-100.
Host
Mouse
Positive Control
NCCIT, NIH/3T3, PC12, Mouse heart, F9, zebrafish brain, Hela HepG2, Mouse brain
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
1-B11
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
50 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:5,000-1:10,000
-
ICC
-
1:200
-
IHC-P
-
1:20
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FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
beta-tubulin
SYNONYMS
Beta 4 tubulin antibody;Beta 5 tubulin antibody;BetaTubulin antibody;TBB5_HUMAN antibody;TUBB antibody;TUBB2 antibody;TUBB2A antibody;TUBB5 antibody;tubulin beta 2A antibody;Tubulin beta chain antibody;Tubulin beta-5 chain antibody
SEQUENCE SIMILARITIES
Belongs to the tubulin family.
TISSUE SPECIFICITY
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
POST-TRANSLATIONAL MODIFICATION
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
SUBCELLULAR LOCATION
Cytoplasm ,cytoskeleton
FUNCTION
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
CITATIONS
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Di Wang
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
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WB
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IP
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mice
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Citation
-
-
Zhang, D. G., Zhao, T., Hog...
Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish. Food chemistry, 360, 129814.
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WB
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Yellow catfish
-
Citation
-
-
Lv, J. J., Wang, H., Cui, H...
Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis. Frontiers in cell and developmental biology, 8, 609090.
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WB
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Mouse
-
Citation
-
-
Guo, Chuansheng et al.
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]
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WB
-
None
-
Citation
-
-
Yi Ting Zhou,Boon Chuan Low
KIF5B transports BNIP-2 to regulate p38 mitogen-activated protein kinase activation and myoblast differentiation
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WB
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mouse
-
Citation
-
-
Zhang, Meng-Yi et al.
Bromodomains and Extra-Terminal (BET) Inhibitor JQ1 Suppresses Proliferation of Acute Lymphocytic Leukemia by Inhibiting c-Myc-Mediated Glycolysis. | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research [2020]
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WB
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human
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Citation
-
-
Sun, Lingling et al.
Regular Swimming Exercise Attenuated Neuroma Pain in Rats: Involvement of Leptin and Adiponectin. | The Journal of Pain : Official Journal of the American Pain Society [2019]
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WB
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rat
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Citation
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