PRODUCT CODE: EM0103

beta-tubulin Mouse Monoclonal Antibody [1-B11] (EM0103)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution.<br />
Positive control: <br />
  Lane 1: NCCIT <br />
  Lane 2: NIH/3T3 <br />
  Lane 3: PC12 <br />
  Lane 4: Mouse heart <br />
  Lane 5: F9 <br />
  Lane 6: zebrafish brain <br />
  Lane 7: Hela
  • Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution.<br />
Positive control: <br />
  Lane 1: NCCIT <br />
  Lane 2: NIH/3T3 <br />
  Lane 3: PC12 <br />
  Lane 4: Mouse heart <br />
  Lane 5: F9 <br />
  Lane 6: zebrafish brain <br />
  Lane 7: Hela
  • ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC stainingβ-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HeLa cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
  • Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/500 dilution.
Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution.
Positive control:
Lane 1: NCCIT
Lane 2: NIH/3T3
Lane 3: PC12
Lane 4: Mouse heart
Lane 5: F9
Lane 6: zebrafish brain
Lane 7: Hela

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

beta-tubulin Mouse Monoclonal Antibody [1-B11] (EM0103)

Immunogen

Peptide

Host

Mouse

Positive Control

NCCIT, NIH/3T3, PC12, Mouse heart, F9, zebrafish brain, Hela HepG2, Mouse brain

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1-B11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • ICC

  • 1:200

  • IHC-P

  • 1:20 FC

TARGET

UNIPROT #

PROTEIN NAME

beta-tubulin

SYNONYMS

Beta 4 tubulin antibody; Beta 5 tubulin antibody; BetaTubulin antibody; TBB5_HUMAN antibody; TUBB antibody; TUBB2 antibody; TUBB2A antibody; TUBB5 antibody; tubulin beta 2A antibody; Tubulin beta chain antibody; Tubulin beta-5 chain antibody

SEQUENCE SIMILARITIES

Belongs to the tubulin family.

TISSUE SPECIFICITY

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

POST-TRANSLATIONAL MODIFICATION

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

SUBCELLULAR LOCATION

Cytoplasm ,cytoskeleton

FUNCTION

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.

CITATIONS

  • Yi Ting Zhou,Boon Chuan Low

    KIF5B transports BNIP-2 to regulate p38 mitogen-activated protein kinase activation and myoblast differentiation

  • Di Wang

    Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation

  • Sun, Lingling et al.

    Regular Swimming Exercise Attenuated Neuroma Pain in Rats: Involvement of Leptin and Adiponectin. | The Journal of Pain : Official Journal of the American Pain Society [2019]

  • Lv, J. J., Wang, H., Cui, H...

    Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis. Frontiers in cell and developmental biology, 8, 609090.

  • Zhang, Meng-Yi et al.

    Bromodomains and Extra-Terminal (BET) Inhibitor JQ1 Suppresses Proliferation of Acute Lymphocytic Leukemia by Inhibiting c-Myc-Mediated Glycolysis. | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research [2020]

  • Zhang, D. G., Zhao, T., Hog...

    Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish. Food chemistry, 360, 129814.

  • Guo, Chuansheng et al.

    Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]