Beta Actin Rabbit Polyclonal Antibody (R1207-1)

  • Zebrafish


  • WB

  • ICC

  • IHC-P

  • FC


  • Human

  • Mouse

  • Rat

  • Zebrafish

  • Bamboo

Western blot analysis of β-actin on different lysates using anti-β-actin antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: NIH/3T3<br />
Lane 2: Hela<br />
Lane 3: PC12<br />
Lane 4: Zebrafish
  • Western blot analysis of β-actin on different lysates using anti-β-actin antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: NIH/3T3<br />
Lane 2: Hela<br />
Lane 3: PC12<br />
Lane 4: Zebrafish
  • Western blot analysis of β-actin on different lysates using anti-β-actin antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: hybrid fish (crucian-carp) brain tissue<br />
Lane 2: hybrid fish (crucian-carp) kidney tissue
Western blot analysis of β-actin on different lysates using anti-β-actin antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: Hela
Lane 3: PC12
Lane 4: Zebrafish


  • WB

  • ICC

  • IHC-P

  • FC


  • Human

  • Mouse

  • Rat

  • Zebrafish

  • Bamboo


Product Type

Rabbit polyclonal primary

Product Name

Beta Actin Rabbit Polyclonal Antibody (R1207-1)


Synthetic peptide within n-terminal residues of β-actin.



Positive Control

Hela, NIH/3T3, PC12, Zebrafish








Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.


1 ug/ul


Immunogen affinity purified






  • WB

  • 1:1,000-5,000




Beta Actin


A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody


Belongs to the actin family.


ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.




Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in Western Blotting.


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