Applications
-
WB
-
IHC-P
REACTIVITY
-
Camellia sinensis
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Auxin response factor Mouse Monoclonal Antibody [14D2] (EM1901-06)
Immunogen
Recombinant protein within tea auxin response factor aa 401-586 / 820.
Host
Mouse
Positive Control
Tea leafbud.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
14D2
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
92 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB:1:1,000-1:2,000
-
IHC-P:1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Auxin response factor
SEQUENCE SIMILARITIES
Belongs to the ARF family.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Auxin response factors (ARFs) are transcription factors that bind to auxin response elements in promoters of early auxin response genes and play central roles in many auxin-mediated processes. As transcription factors, ARFs possess a modular structure and consist of three major domains: an amino-terminal DNA-binding domain (DBD); a middle domain, which is responsible for gene activation or repression; and a carboxy-terminal PB1(Phox and Bem1) domain, contained within a region that was previously called domain III/I. ARFs function as transcription activators or repressors, depending on the middle region of the ARF protein; Q-rich middle regions activate transcription, whereas P/S/T-rich middle regions confer repressor activity.