AU5 tag Rabbit Polyclonal Antibody
Catalog# R1402-4
AU5 tag Rabbit Polyclonal Antibody
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WB
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ELISA
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Species independent
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unconjugated
Overview
Product Name
AU5 tag Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein fused with AU5 Tag.
Species Reactivity
Species independent
Validated Applications
WB, ELISA
Positive Control
AU5 tagged fusion protein
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5,000
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ELISA
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1:10,000
Target
Function
AU5 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.
Background References
暂无
Synonyms
AU5 epitope tag antibody
TDFYLK epitope tag antibody
TDFYLK tag antibody
Images
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Western blot analysis of AU5 tag on AU5 tagged fusion protein with Rabbit anti-AU5 tag antibody (R1402-4) at 1/1,000 dilution.
Lane 1: AU5 tagged fusion protein, 1,000ng/Lane
Lane 2: AU5 tagged fusion protein, 200ng/Lane
Lane 3: AU5 tagged fusion protein, 50ng/Lane
Exposure time: 51 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1402-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"