PRODUCT CODE: ER1802-84

ASK1 Rabbit Polyclonal Antibody (ER1802-84)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of ASK1 on human skeletal muscle tissue lysate using anti-ASK1 antibody at 1/500 dilution.
  • Western blot analysis of ASK1 on human skeletal muscle tissue lysate using anti-ASK1 antibody at 1/500 dilution.
  • ICC staining ASK1 (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
Western blot analysis of ASK1 on human skeletal muscle tissue lysate using anti-ASK1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ASK1 Rabbit Polyclonal Antibody (ER1802-84)

Immunogen

Synthetic peptide of c-terminal human ask1.

Host

Rabbit

Positive Control

Human skeletal muscle tissue lysate, human colon cancer tissue, mouse testis tissue, PANC-1.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

155 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ASK1

SYNONYMS

Apoptosis signal regulating kinase 1 antibody; Apoptosis signal-regulating kinase 1 antibody; ASK 1 antibody; ASK-1 antibody; ASK1 antibody; M3K5 antibody; M3K5_HUMAN antibody; MAP/ERK kinase kinase 5 antibody; MAP3K5 antibody; MAPK/ERK kinase kinase 5 antibody; MAPKKK5 antibody; MEK kinase 5 antibody; MEKK 5 antibody; MEKK5 antibody; Mitogen activated protein kinase kinase kinase 5 antibody; Mitogen-activated protein kinase kinase kinase 5 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

TISSUE SPECIFICITY

Abundantly expressed in heart and pancreas.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1 and AKT2. Phosphorylated at Ser-966 which induces association of MAP3K5/ASK1 with the 14-3-3 family proteins and suppresses MAP3K5/ASK1 activity. Calcineurin (CN) dephosphorylates this site. Also dephosphorylated and activated by PGAM5.; Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination leading to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm. Endoplasmic reticulum.

FUNCTION

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.