Applications
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Apg7 Rabbit Polyclonal Antibody (ER1802-18)
Immunogen
Synthetic peptide within human apg7 aa 541-590 / 703.
Host
Rabbit
Positive Control
A431, A549, HUVEC, human breast tissue, human placenta tissue, mouse kidney tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
78 kDa
Isotype
IgG
APPLICATION DILUTION
-
ICC
-
1:500-1:2,000
-
IHC-P
-
1:200-1:1,000
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Apg7
SYNONYMS
1810013K23Rik antibody; Apg 7 antibody; APG7 autophagy 7 like antibody; APG7 autophagy 7-like (S. cerevisiae) antibody; APG7 like antibody; APG7, S. cerevisiae, homolog of antibody; APG7-like antibody; APG7L antibody; ATG 7 antibody; ATG12-activating enzyme E1 ATG7 antibody; ATG7 antibody; ATG7 autophagy related 7 homolog (S. cerevisiae) antibody; ATG7 autophagy related 7 homolog antibody; ATG7_HUMAN antibody; Atg7l antibody; Autophagy 7, S. cerevisiae, homolog of antibody; Autophagy related protein 7 antibody; Autophagy-related 7 (yeast) antibody; Autophagy-related protein 7 antibody; DKFZp434N0735 antibody; GSA 7 antibody; GSA7 antibody; hAGP7 antibody; Ubiquitin activating enzyme E1 like protein antibody; Ubiquitin-activating enzyme E1-like protein antibody; Ubiquitin-like modifier-activating enzyme ATG7 antibody
SEQUENCE SIMILARITIES
Belongs to the ATG7 family.
TISSUE SPECIFICITY
Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
POST-TRANSLATIONAL MODIFICATION
Acetylated by EP300.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy.