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PRODUCT CODE: ER1802-18

Apg7 Rabbit Polyclonal Antibody (ER1802-18)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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ICC staining Apg7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Apg7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Apg7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Apg7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with Apg7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
ICC staining Apg7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Apg7 Rabbit Polyclonal Antibody (ER1802-18)

Immunogen

Synthetic peptide within human apg7 aa 529-592.

Host

Rabbit

Positive Control

A431, A549, HUVEC, human breast tissue, human placenta tissue, mouse kidney tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

78 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:500-1:2,000

  • IHC-P

  • 1:200-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

O95352

PROTEIN NAME

Apg7

SYNONYMS

1810013K23Rik antibody; Apg 7 antibody; APG7 autophagy 7 like antibody; APG7 autophagy 7-like (S. cerevisiae) antibody; APG7 like antibody; APG7, S. cerevisiae, homolog of antibody; APG7-like antibody; APG7L antibody; ATG 7 antibody; ATG12-activating enzyme E1 ATG7 antibody; ATG7 antibody; ATG7 autophagy related 7 homolog (S. cerevisiae) antibody; ATG7 autophagy related 7 homolog antibody; ATG7_HUMAN antibody; Atg7l antibody; Autophagy 7, S. cerevisiae, homolog of antibody; Autophagy related protein 7 antibody; Autophagy-related 7 (yeast) antibody; Autophagy-related protein 7 antibody; DKFZp434N0735 antibody; GSA 7 antibody; GSA7 antibody; hAGP7 antibody; Ubiquitin activating enzyme E1 like protein antibody; Ubiquitin-activating enzyme E1-like protein antibody; Ubiquitin-like modifier-activating enzyme ATG7 antibody

SEQUENCE SIMILARITIES

Belongs to the ATG7 family.

TISSUE SPECIFICITY

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

POST-TRANSLATIONAL MODIFICATION

Acetylated by EP300.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy.