Anti-DYKDDDDK (Flag) Magnetic Beads
Catalog# HAK21011
Anti-DYKDDDDK (Flag) Magnetic Beads
Overview
Product Description
产品包装:
|
产品编号 |
产品名称 |
产品形式 |
包装规格(V/V, 1/5) |
|
HAK21011-1 mL |
Anti-DYKDDDDK Magnetic Beads |
20% 磁珠悬液 |
1 mL |
|
HAK21011-5 mL |
Anti-DYKDDDDK Magnetic Beads |
20% 磁珠悬液 |
5 mL |
|
HAK21011-25 mL |
Anti-DYKDDDDK Magnetic Beads |
20% 磁珠悬液 |
25 mL |
产品描述:
Anti-DYKDDDDK 免疫磁珠来源于 Anti-DYKDDDDK [A2-A4]重组鼠单克隆抗体,与直径为 30-150 μm 的琼脂糖磁珠共价偶联。 [A2-A4]抗体能识别包含 Flag 多肽序列(DYKDDDDK)的融合蛋白。[A2-A4]可特异性地结合哺乳动物和细菌提取物中的甲硫氨酸修饰的 N 端 Flag 融合蛋白、C 端 Flag 融合蛋白。
Anti-DYKDDDDK 免疫磁珠可用于常用的免疫沉淀程序检测和捕获 Flag 标记的融合蛋白。可以通过磁性吸附实现快速高效的分离,无需离心操作,以促进实验过程。例如重复洗涤或处理多孔板中的多个样品。
Product Features
Product Performance
产品形式:Anti-DYKDDDDK 免疫磁珠保存在 PBS,pH7.4 添加防腐剂的缓冲液中,以 20%的悬浮液形式提供。
结合能力:每 1mL 纯磁珠可结合≥0.6mg Flag 融合蛋白,测试蛋白C 端融合 Flag 标签,分子量为 70KDa 的蛋白。
表 1 磁珠结合能力
|
纯磁珠的体积(ul) |
结合能力(ug) |
|
20 |
〜12 |
|
50 |
〜30 |
|
100 |
〜60 |
|
200 |
〜120 |
注:不要使用磁力搅拌系统。磁力搅拌系统会破坏磁珠结构。
辅助设备:磁力架、离心管、96 孔板。Storage Instructions
Anti-DYKDDDDK免疫磁珠采用蓝冰运输。建议在2-8℃下储存,可稳定保存2年。请勿-20℃保存,冷冻磁珠将不可逆转地损坏磁珠结构。
Images
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Immunoprecipitation
Anti-DYKDDDDK [A2-A4] Magnetic Beads: Cat.No. HAK21011: Purification of Two Different FLAG-tagged fusion protein from HeLa transfected with Flag-fusion protein expression vector. 10ul Anti-FLAG [A2-A4] Magnetic Beads was used for IP per lane.
Lane 1, 2, 3, 4: Hela transfected with Flag-fusion protein expression vector containing an N-terminal Flag tag, whole cell lysate
Lane 1’, 2’, 3’, 4’: Hela transfected with Flag-fusion protein expression vector containing an C-terminal Flag tag, whole cell lysate -
Immunoprecipitation
Lane 1, 2, 3, 4, 5: Input, 5μL, 10μL, 15μL, 20 μL Anti-DYKDDDDK [A2-A4] Magnetic Beads suspension were add to the same volume of the Hela lysate transfected with Flag-fusion protein expression vector containing an N-terminal Flag tag, whole cell lysate.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Bombyx mori ER Chaperones Calnexin and Calreticulin Promote BmNPV Infection Through Facilitating GP64 Expression
Journal: Archives Of Insect Biochemistry And Physiology
DOI: 10.1002/arch.70129
IF: 1.9
Application: CoIP
Reactivity: Silkworm
Publish date: 2026 Feb
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Oridonin Ameliorates Alzheimer's Disease-Like Pathology in Male Mice Through Inhibition of Receptor-Interacting Protein Kinase 1
Journal: Phytotherapy Research
DOI: 10.1002/ptr.70185
IF: 6.3
Application:
Reactivity:
Publish date: 2026 Feb
-
Histone Deacetylase 6 Inhibits STING-Dependent Antiviral Immunity via Site-Specific Deacetylation
Journal: Journal Of Biological Chemistry
DOI: 10.1016/j.jbc.2025.110841
IF: 3.9
Application: IP
Reactivity:
Publish date: 2025 Oct
-
Sciellin inhibits senescence and promotes pancreatic cancer progress by activating the notch signaling pathway
Journal: Scientific Reports
DOI: 10.1038/s41598-025-88265-0
IF: 3.8
Application: CoIP
Reactivity: Human
Publish date: 2025 May
-
YTHDC1 negatively regulates UBE3A to influence RAD51 ubiquitination and inhibit apoptosis in colorectal cancer cells
Journal: Scientific Reports
DOI: 10.1038/s41598-025-92925-6
IF: 3.8
Application: Co-IP
Reactivity: Human
Publish date: 2025 Mar
-
The F box protein INHIBITOR FOR BROWN FURROWS 1 (IBF1) regulates flavonoid accumulation in rice hull by promoting degradation of chalcone synthase 1
Journal: Plant Journal
DOI: 10.1111/tpj.70105
IF: 6.2
Application:
Reactivity:
Publish date: 2025 Mar
-
Macrophages and macrophage extracellular vesicles confer cancer ferroptosis resistance via PRDX6-mediated mitophagy inhibition
Journal: Redox Biology
DOI: 10.1016/j.redox.2025.103826
IF: 11.9
Application: WB
Reactivity: Mouse
Publish date: 2025 Aug
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The NR3C2-SIRT1 signaling axis promotes autophagy and inhibits epithelial mesenchymal transition in colorectal cancer
Journal:
DOI: 10.1038/s41419-025-07575-3
IF:
Application: WB
Reactivity: Mouse
Publish date: 2025 Apr
-
SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice
Journal: Cell Death & Differentiation
DOI:
IF: 13.7
Application: IP
Reactivity: Mouse
Publish date: 2024 Oct
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