alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY02-64] (ET1607-53)
Catalog# ET1607-53
Rabbit Monoclonal to alpha smooth muscle Actin
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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mIHC
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Human
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Mouse
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Rat
Lane 1: Saos-2 cell lysate (15 µg/Lane)
Lane 2: A431 cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: C2C12 cell lysate (15 µg/Lane)
Lane 5: Neuro-2a cell lysate (15 µg/Lane)
Lane 6: Mouse skin tissue lysate (30 µg/Lane)
Lane 7: Rat skin tissue lysate (30 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 46 seconds
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-53) at 1/20,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY02-64] (ET1607-53)
Immunogen
Synthetic peptide within n-terminal human alpha smooth muscle actin.
Host
Rabbit
Positive Control
Saos-2 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, mouse skin tissue lysate, rat skin tissue lysate, human breast carcinoma tissue, HeLa cell lysate, A431 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, L6 cell lysate, mouse heart tissue lysate, mouse skin tissue lysate, rat heart tissue lysate, rat skin tissue lysate, rat smooth muscle tissue lysate, human kidney tissue, human stomach cancer tissue, human pancreatic carcinoma, HepG2, human tonsil tissue, human liver tissue, human gastric cancer, Human non-small cell lung cancer, mouse pancreas, mouse liver, mouse kidney, human stomach cancer tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue, NIH/3T3, PC-12, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY02-64
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:50,000
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IF-Cell
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1:2,000
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IF-Tissue
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1:1,000
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IHC-P
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1:50,000
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FC
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1:1,000-1:2,000
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mIHC
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1:2,000-1:10,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
alpha smooth muscle Actin
SYNONYMS
alpha SMA;a-SMA antibody;asma antibody;a actin antibody;AAT6 antibody;ACTA_HUMAN antibody;ACTA2 antibody;Actin alpha 2 smooth muscle aorta antibody;Actin aortic smooth muscle antibody;Actin, aortic smooth muscle antibody;ACTSA antibody;ACTVS antibody;Alpha 2 actin antibody;Alpha actin 2 antibody;Alpha cardiac actin antibody;Alpha-actin-2 antibody;Cell growth inhibiting gene 46 protein antibody;Cell growth-inhibiting gene 46 protein antibody;GIG46 antibody;Growth inhibiting gene 46 antibody;MYMY5 antibody
SEQUENCE SIMILARITIES
Belongs to the actin family.
POST-TRANSLATIONAL MODIFICATION
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.; Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-75 by SETD3.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
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