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AKT1 Recombinant Rabbit Monoclonal Antibody [ST05-09] (ET1609-47)

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Catalog# ET1609-47

Rabbit Monoclonal to AKT1

Tested for

  • WB

  • IHC-P

  • IP

  • FC

  • IHC-Fr

  • IF-Cell

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

AKT1 Recombinant Rabbit Monoclonal Antibody [ST05-09] (ET1609-47)

Immunogen

Synthetic peptide within c-terminal human akt1.

Host

Rabbit

Positive Control

HeLa cell lysate, A549 cell lysate, C6 cell lysate, PC-12 cell lysate, SH-SY5Y cell lysates, mouse brain tissue, mouse kidney tissue, human kidney tissue, mouse prostate tissue, Hela, mouse hippocampus tissue, mouse cerebral cortex tissue, MCF7, C6.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST05-09

RRID

AB_3069857

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:50-1:5,000

  • FC

  • 1:50-1:100

  • IP

  • 1-2μg/sample

  • IHC-Fr

  • 1:100

  • IF-Cell

  • 1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 56 kDa

Isotype

IgG

TARGET

UNIPROT #

P31749

PROTEIN NAME

AKT1

SYNONYMS

AKT 1 antibody;AKT antibody;AKT1 antibody;AKT1_HUMAN antibody;MGC99656 antibody;PKB antibody;PKB-ALPHA antibody;PRKBA antibody;Protein Kinase B Alpha antibody;Protein kinase B antibody;Proto-oncogene c-Akt antibody;RAC Alpha antibody;RAC antibody;RAC-alpha serine/threonine-protein kinase antibody;RAC-PK-alpha antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.

TISSUE SPECIFICITY

Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.

POST-TRANSLATIONAL MODIFICATION

O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site.; Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Phosphorylated at Thr-308 and Ser-473 by IKBKE and TBK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells. Ser-473 phosphorylation is enhanced by signaling through activated FLT3 (By similarity). Ser-473 is dephosphorylated by PHLPP. Dephosphorylated at Thr-308 and Ser-473 by PP2A phosphatase. The phosphorylated form of PPP2R5B is required for bridging AKT1 with PP2A phosphatase. Ser-473 is dephosphorylated by CPPED1, leading to termination of signaling.; Ubiquitinated via 'Lys-48'-linked polyubiquitination by ZNRF1, leading to its degradation by the proteasome (By similarity). Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. Also ubiquitinated by TRIM13 leading to its proteasomal degradation. Phosphorylated, undergoes 'Lys-48'-linked polyubiquitination preferentially at Lys-284 catalyzed by MUL1, leading to its proteasomal degradation.; Acetylated on Lys-14 and Lys-20 by the histone acetyltransferases EP300 and KAT2B. Acetylation results in reduced phosphorylation and inhibition of activity. Deacetylated at Lys-14 and Lys-20 by SIRT1. SIRT1-mediated deacetylation relieves the inhibition.

SUBCELLULAR LOCATION

Cell membrane, Cytoplasm, Membrane, Nucleus.

FUNCTION

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβor RacPK-β) and Akt 3 (also designated PKB? or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

CITATIONS

  • Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3

  • Jian-Li Jiang,Hong-Yong Cu,Zhi-Nan Chen

    Hypo-phosphorylated CD147 promotes migration and invasion of hepatocellular carcinoma cells and predicts a poor prognosis. Cellular oncology (Dordrecht), 42(4), 537–554.

    • WB

    • CO-IP

    • IHC

    • IF

    • Human hepatocellular carcinoma cell

    • Citation

  • Tang, Y., Jin, Y. H., Li, H. L., Xin, H., Chen, J. D., Li, X. Y., & Pan, Y. F.

    PBRM1 deficiency oncogenic addiction is associated with activated AKT–mTOR signalling and aerobic glycolysis in clear cell renal cell carcinoma cells

  • Haoyan Wu,et al

    A Multifunctional miRNA Delivery System Based on Tetrahedral Framework Nucleic Acids for Regulating Inflammatory Periodontal Ligament Stem Cells and Attenuating Periodontitis Bone Loss

  • Yao Peixue,et al

    IL-17A regulates collagen synthesis after autophagy in bronchial fibroblasts through the PI3K/AKT/mTOR pathway

  • Fan, R., Chen, C., Hu, J., Mu, M., Chuan, D., Chen, Z., Guo, G., & Xu, J.

    Multifunctional gold nanorods in low-temperature photothermal interactions for combined tumor starvation and RNA interference therapy

  • Shusen Zheng

    Combination of HSP90 and autophagy inhibitors promotes hepatocellular carcinoma apoptosis following incomplete thermal ablation

  • Wanshun Wang,et al

    Microenvironment-responsive injectable hydrogel for neuro-vascularized bone regeneration

  • Falong Yang,et al

    Luteolin-mediated phosphoproteomic changes in chicken splenic lymphocytes: unraveling the detoxification mechanisms against ammonia-induced stress

  • Gao XK, Sheng ZK, Lu YH, et al

    VAPB-mediated ER-targeting stabilizes IRS-1 signalosomes to regulate insulin/IGF signaling

  • Jiang Huangyu,et al

    Evodiamine Inhibits the Progression of Esophageal Aquamous Cell Carcinoma via Modulating PI3K/AKT/mTOR Pathway

  • Koda, S., Zhang, B., Zhou, Q. Y., Xu, N., Li, J., Liu, J. X., Liu, M., Lv, Z. Y., Wang, J. L., Shi, Y., Gao, S., Yu, Q., Li, X. Y., Xu, Y. H., Chen, J. X., Tekengne, B., Adzika, G. K., Tang, R. X., Sun, H., Zheng, K. Y., … Yan, C.

    β2-Adrenergic Receptor Enhances the Alternatively Activated Macrophages and Promotes Biliary Injuries Caused by Helminth Infection

  • Yue Yaru,et al

    Rational formulation of saponin and dexamethasone for the synergistic treatment of rheumatoid arthritis in vivo

  • Li Zong,et al

    Schisandrin A alleviates non-alcoholic fatty liver disease by improving liver metabolic disorders and suppressing the GSK3β signaling pathway

  • Song, X., Liu, B., Zhao, G., Pu, X., Liu, B., Ding, M., & Xue, Y.

    Streptococcus pneumoniae promotes migration and invasion of A549 cells in vitro by activating mTORC2/AKT through up-regulation of DDIT4 expression

  • Cao, X., Shu, Y., Chen, Y., Xu, Q., Guo, G., Wu, Z., Shao, M., Zhou, Y., Chen, M., Gong, Y., Li, C., Shi, Y., & Bu, H.

    Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.

  • Weikang Zou,et al

    Role of HDAC3 in the epithelial-mesenchymal transition of retinal pigment epithelium cells: implications for proliferative vitreoretinopathy

  • He, J., Lin, M., Zhang, X., Zhang, R., Tian, T., Zhou, Y., Dong, W., Yang, Y., Sun, X., Dai, Y., Xu, Y., Zhang, Z., Xu, M., Lei, Q. Y., Xu, Y., & Lv, L.

    TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential

  • Meng, Y., Hu, X., Li, S., Zeng, X., Qiu, L., Wei, M., Wang, Z., & Han, J.

    miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1. BMC cancer, 20(1), 1028.

  • Jingmin Zhou,Junbo Ge

    Angiotensin II induces apoptosis of cardiac microvascular endothelial cells via regulating PTP1B/PI3K/Akt pathway.

  • Li, J., Zhang, X., Guo, D., Shi, Y., Zhang, S., Yang, R., & Cheng, J.

    The mechanism of action of paeoniae radix rubra-angelicae sinensis radix drug pair in the treatment of rheumatoid arthritis through PI3K/AKT/NF-κB signaling pathway

  • Han Liu , Haiyang Guo , Yuehan Wu , et al

    RCN1 deficiency inhibits oral squamous cell carcinoma progression and THP-1 macrophage M2 polarization

  • Jin Mei,et al

    Screening and bioinformatics analysis of a potential ceRNA network in melatonin-induced cashmere growth in Liaoning cashmere goats

  • Apatinib Inhibits Bladder Cancer through Suppression of the VEGFR2-PI3K-AKT Signaling Pathway as Revealed by Network Pharmacology and in vitro Experimental Verification

  • Jing Zhang;Jin-Shui Zhu

    Network pharmacology-based identification of the antitumor effects of taraxasterol in gastric cancer

  • Yang Yanfang,et al

    SUMOylation of annexin A6 retards cell migration and tumor growth by suppressing RHOU/AKT1-involved EMT in hepatocellular carcinoma

  • Xianlei Han,et al

    Network pharmacology and phytochemical composition combined with validation in vivo and in vitro reveal the mechanism of platycodonis radix ameliorating PM2.5-induced acute lung injury

  • Hou Xiaorong

    Dipeptide PA3264 derived from rare and endangered Squama Manis is a novel bioactive peptide for the treatment of triple-negative breast cancer

  • Gao, X. K., Rao, X. S., Cong, X. X., Sheng, Z. K., Sun, Y. T., Xu, S. B., Wang, J. F., Liang, Y. H., Lu, L. R., Ouyang, H., Ge, H., Guo, J. S., Wu, H. J., Sun, Q. M., Wu, H. B., Bao, Z., Zheng, L. L., & Zhou, Y. T.

    Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes

  • Yue Xiao,et al

    Dietary oxygenated water mitigates type 2 diabetes mellitus by modulating gut microbiota and enhancing glucose metabolism in skeletal muscle

  • Luo, C., Zhou, M., Chen, C., Li, S., Li, Q., Huang, Y., & Zhou, Z.

    A liposome-based combination strategy using doxorubicin and a PI3K inhibitor efficiently inhibits pre-metastatic initiation by acting on both tumor cells and tumor-associated macrophages

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