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Zfp352 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-55

Zfp352 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Zfp352 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse Zfp352 aa 100-150.

Species Reactivity

Mouse

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 62 kDa

Positive Control

GFP-tagged Zfp352 transfected 293T cells lysate, GFP-tagged Zfp352 transfected 293F cells lysate.

Conjugation

unconjugated

RRID

AB_3069532

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

Target

Function

Zfp352 (formerly 2czf48), that is expressed in early mouse embryos. The Zfp352 gene is intron‐less in the coding region but carries a solitary 4.3‐kb intron in the 5′‐untranslated region.

Background References

1. Chen HH. Generation of two homologous and intronless zinc-finger protein genes, zfp352 and zfp353, with different expression patterns by retrotransposition. Genomics. 2002 Jan;79(1):18-23.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: A2AML7 Mouse

Synonyms

ZZfp352 antibody

Zinc finger protein 352 antibody

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Zfp352 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-55" style="font-weight: bold;text-decoration: underline;">ER2001-55</a>, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control:<br />Lane 1: Non-transfected 293T cells lysate<br />Lane 2: GFP-tagged Zfp352 transfected 293T cells lysate<br />Lane 3: Non-transfected 293F cells lysate<br />Lane 4: GFP-tagged Zfp352 transfected 293F cells lysate

    ☑ Cell treatment (CT)

    Western blot analysis of Zfp352 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-55, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Non-transfected 293T cells lysate
    Lane 2: GFP-tagged Zfp352 transfected 293T cells lysate
    Lane 3: Non-transfected 293F cells lysate
    Lane 4: GFP-tagged Zfp352 transfected 293F cells lysate

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />ICC staining of Zfp352 in Non-transfected 293T cells and GFP-tagged Zfp352 transfected 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ER2001-55" style="font-weight: bold;text-decoration: underline;">ER2001-55</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ☑ Cell treatment (CT)

    ICC staining of Zfp352 in Non-transfected 293T cells and GFP-tagged Zfp352 transfected 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"