Vang-like protein 1 Rabbit Polyclonal Antibody
Catalog# 0905-5
Vang-like protein 1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
Vang-like protein 1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human Vang-like protein 1 aa 114-171.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 60 kDa
Positive Control
Hela, MCF-7, LOVO.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:100-1:200
Target
Function
Vang-like protein 1 is a multi-pass membrane protein, expressed specifically in testis and ovary. It interacts through its C-terminal region with the N-terminal half of DVL1, DVL2 and DVL3. Vangl proteins play a key developmental role in establishing planar cell polarity (PCP) and in regulating convergent extension (CE) movements during embryogenesis. Vangl1 and Vangl2 are both downregulated in several cancer cell lines and primary tumors. Defects in VANGL1 are a cause of neural tube defects (NTD) and a cause of sacral defect with anterior meningocele (SDAM).
Background References
1. Kibar Z et al. Mutations in VANGL1 associated with neural-tube defects. N Engl J Med 356:1432-1437 (2007).
2. Kibar Z et al. Novel mutations in VANGL1 in neural tube defects. Hum Mutat 30:E706-E715 (2009).
Sequence Similarity
Belongs to the Vang family.
Tissue Specificity
According to PubMed:11956595, ubiquitously expressed. According to PubMed:12011995, expressed specifically in testis and ovary.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
ANGL planar cell polarity protein 1 antibody
KAI1 C-terminal interacting tetraspanin antibody
KITENIN antibody
Loop tail protein 2 homolog antibody
Loop-tail protein 2 homolog antibody
LPP2 antibody
MGC5338 antibody
STB2 antibody
STBM2 antibody
Strabismus 2 antibody
ExpandImages
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Western blot analysis of VANGL1 on MCF-7 cell lysate using anti-VANGL1 antibody at 1/500 dilution.
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ICC staining VANGL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining VANGL1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining VANGL1in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"