USP29 Rabbit Polyclonal Antibody
Catalog# ER1902-76
USP29 Rabbit Polyclonal Antibody
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
USP29 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human USP29 aa 665-922 / 922.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 104 kDa
Positive Control
Rat testis tissue lysate, mouse testis tissue lysate, 293.
Conjugation
unconjugated
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | |
|---|---|
| mouse |
|
| rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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FC
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1:50-1:100
Target
Function
The DNA damage checkpoint is essential for the maintenance of genome integrity after genotoxic stress, and also for cell survival in eukaryotes. Claspin has a key role in the ATR (ATM and Rad3-related)-Chk1 branch of the DNA damage checkpoint and is also required for correct DNA replication. To achieve properly these functions, Claspin is tightly regulated by ubiquitinin-dependent proteasomal degradation, which controls Claspin levels in a DNA-damage- and cell-cycle-dependent manner. USP29 is a regulator of Claspin, USP29 is a player in the ATR-Chk1 pathway and the control of DNA replication.
Background References
1. Martín Y et al. USP29 controls the stability of checkpoint adaptor Claspin by deubiquitination. Oncogene 34(8):1058-1063 (2015).
Sequence Similarity
Belongs to the peptidase C19 family.
Subcellular Location
Cytoplasm, perinuclear region.
Synonyms
Deubiquitinating enzyme 29 antibody
HOM TES 84/86 antibody
MGC163266 antibody
MGC163270 antibody
Ubiquitin carboxyl terminal hydrolase 29 antibody
Ubiquitin carboxyl-terminal hydrolase 29 antibody
Ubiquitin specific peptidase 29 antibody
Ubiquitin specific processing protease 29 antibody
Ubiquitin specific processing protease antibody
Ubiquitin thiolesterase 29 antibody
ExpandImages
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Western blot analysis of USP29 on different lysates with Rabbit anti-USP29 antibody (ER1902-76) at 1/500 dilution.
Lane 1: Rat testis tissue lysate
Lane 2: Mouse testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-76) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of USP29 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-76, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"