Topoisomerase II alpha Rabbit Polyclonal Antibody

Function

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. It is essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Background References

1. "Use of divalent metal ions in the DNA cleavage reaction of human type II topoisomerases." Deweese J.E., Burch A.M., Burgin A.B., Osheroff N. Biochemistry 48:1862-1869(2009)

2. "The structure of DNA-bound human topoisomerase II alpha: conformational mechanisms for coordinating inter-subunit interactions with DNA cleavage." Wendorff T.J., Schmidt B.H., Heslop P., Austin C.A., Berger J.M. J. Mol. Biol. 424:109-124(2012)

Sequence Similarity

Belongs to the type II topoisomerase family.

Post-translational Modification

Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

Subcellular Location

Cytoplasm, nucleus.

UNIPROT

SwissProt: P11388 Human

Synonyms