Thymidine Phosphorylase Mouse Monoclonal Antibody [A1A8]
Catalog# EM1901-23
Thymidine Phosphorylase Mouse Monoclonal Antibody [A1A8]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Thymidine Phosphorylase Mouse Monoclonal Antibody [A1A8]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Thymidine Phosphorylase aa 19-239 / 482.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 50 kDa
Positive Control
THP-1 cell lysate, A549 cell lysate, A431 cell lysate, human liver carcinoma tissue, human appendix tissue, human spleen tissue.
Conjugation
unconjugated
Clone Number
A1A8
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
Target
Function
Platelet-derived endothelial cell growth factor (PD-ECGF), which is alternatively designated thymidine phosphorylase or gliostatin, is an angiogenic inducer that potently stimulates the growth of endothelial cells and induces chemotaxis. Biologically active PD-ECGF is a functional dimer that consists of two single polypeptide chains that are expressed in platelets, placenta, foreskin fibroblasts and various squamous cell carcinomas, and they are slowly secreted from the cells. In addition, PD-ECGF is overexpressed in tumor and lesional psoriatic skin and lesional epidermis, indicating that it may play a role in the pathophysiology of psoriasis. Serine residues of PD-ECGF are frequently associated with nucleotide triphosphates, including ATP. In an ATP dependent manner, PD-ECGF is also able to catalyze the reversible phosphorolysis of thymidine to thymine, as it contains thymidine phosphorylase activities.
Background References
1. Chapouly C. et. al. Astrocytic TYMP and VEGFA drive blood-brain barrier opening in inflammatory central nervous system lesions. Brain. 2015 Jun;138(Pt 6):1548-67.
2. Sadahiro S. et. al. Increase in Gene Expression of TYMP, DPYD and HIF1A Are Associated with Response to Preoperative Chemoradiotherapy Including S-1 or UFT for Rectal Cancer. Anticancer Res. 2016 May;36(5):2433-40.
Sequence Similarity
Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Subcellular Location
Cytosol.
UNIPROT
Synonyms
ECGF 1 antibody
ECGF antibody
ECGF1 antibody
Endothelial cell growth factor 1 antibody
Endothelial cell growth factor 1 platelet derived antibody
Endothelial cell growth factor, platelet-derived antibody
Gliostatin antibody
hPD ECGF antibody
MEDPS1 antibody
MNGIE antibody
ExpandImages
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Western blot analysis of Thymidine Phosphorylase on different lysates with Mouse anti-Thymidine Phosphorylase antibody (EM1901-23) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: A549 cell lysate
Lane 3: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-23) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"