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Thymidine Phosphorylase Mouse Monoclonal Antibody [A1A10]

Usd: 350

Specification

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Catalog# EM1901-22

Thymidine Phosphorylase Mouse Monoclonal Antibody [A1A10]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Thymidine Phosphorylase Mouse Monoclonal Antibody [A1A10]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human Thymidine Phosphorylase aa 19-239 / 482.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 50 kDa

Positive Control

THP-1 cell lysate, A549 cell lysate, A431 cell lysate, HeLa cell lysate, human liver carcinoma tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

A1A10

RRID

AB_3068867

Product Features

Form

Liquid

Concentration

2 mg/mL(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:200-1:10,000

Target

Function

Platelet-derived endothelial cell growth factor (PD-ECGF), which is alternatively designated thymidine phosphorylase or gliostatin, is an angiogenic inducer that potently stimulates the growth of endothelial cells and induces chemotaxis. Biologically active PD-ECGF is a functional dimer that consists of two single polypeptide chains that are expressed in platelets, placenta, foreskin fibroblasts and various squamous cell carcinomas, and they are slowly secreted from the cells. In addition, PD-ECGF is overexpressed in tumor and lesional psoriatic skin and lesional epidermis, indicating that it may play a role in the pathophysiology of psoriasis. Serine residues of PD-ECGF are frequently associated with nucleotide triphosphates, including ATP. In an ATP dependent manner, PD-ECGF is also able to catalyze the reversible phosphorolysis of thymidine to thymine, as it contains thymidine phosphorylase activities.

Background References

1. Chapouly C. et. al. Astrocytic TYMP and VEGFA drive blood-brain barrier opening in inflammatory central nervous system lesions. Brain. 2015 Jun;138(Pt 6):1548-67.

2. Sadahiro S. et. al. Increase in Gene Expression of TYMP, DPYD and HIF1A Are Associated with Response to Preoperative Chemoradiotherapy Including S-1 or UFT for Rectal Cancer. Anticancer Res. 2016 May;36(5):2433-40.

Sequence Similarity

Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Subcellular Location

Cytosol.

UNIPROT

SwissProt: P19971 Human

SwissProt: Q99N42 Mouse

SwissProt: Q5FVR2 Rat

Synonyms

ECGF 1 antibody

ECGF antibody

ECGF1 antibody

Endothelial cell growth factor 1 antibody

Endothelial cell growth factor 1 platelet derived antibody

Endothelial cell growth factor, platelet-derived antibody

Gliostatin antibody

hPD ECGF antibody

MEDPS1 antibody

MNGIE antibody

Expand

Images

  • Western blot analysis of Thymidine Phosphorylase on different lysates with Mouse anti-Thymidine Phosphorylase antibody (<a href="/products/EM1901-22" style="font-weight: bold;text-decoration: underline;">EM1901-22</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: A431 cell lysate<br />Lane 4: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 25 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1901-22" style="font-weight: bold;text-decoration: underline;">EM1901-22</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Thymidine Phosphorylase on different lysates with Mouse anti-Thymidine Phosphorylase antibody (EM1901-22) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: A431 cell lysate
    Lane 4: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 25 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-22" style="font-weight: bold;text-decoration: underline;">EM1901-22</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-22" style="font-weight: bold;text-decoration: underline;">EM1901-22</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-Thymidine Phosphorylase antibody (<a href="/products/EM1901-22" style="font-weight: bold;text-decoration: underline;">EM1901-22</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-22" style="font-weight: bold;text-decoration: underline;">EM1901-22</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-Thymidine Phosphorylase antibody (EM1901-22) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-22) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-Thymidine Phosphorylase antibody (<a href="/products/EM1901-22" style="font-weight: bold;text-decoration: underline;">EM1901-22</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-22" style="font-weight: bold;text-decoration: underline;">EM1901-22</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-Thymidine Phosphorylase antibody (EM1901-22) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-22) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"