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TRAF6 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1311-2

TRAF6 Rabbit Polyclonal Antibody

Application

  • IF-Cell

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Chicken

  • Zebrafish

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TRAF6 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within C-terminal human TRAF6.

Species Reactivity

Human, Mouse, Rat (Predicted: Chicken, Zebrafish)

Predicted species support after-sales service

Validated Applications

IF-Cell, WB

Molecular Weight

Predicted band size: 60 kDa

Positive Control

CTLL-2, EL4 , PC-12, L6, HeLa, hybrid fish (crucian-carp) heart tissue lysate.

Conjugation

unconjugated

RRID

AB_3073257

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:100-1:200

Target

Function

TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/TRANCE/RANKL and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines.

Background References

1. "Association of beta-arrestin and TRAF6 negatively regulates Toll-like receptor-interleukin 1 receptor signaling." Wang Y., Tang Y., Teng L., Wu Y., Zhao X., Pei G. Nat. Immunol. 7:139-147(2006)

2. "The type I TGF-beta receptor engages TRAF6 to activate TAK1 in a receptor kinase-independent manner." Sorrentino A., Thakur N., Grimsby S., Marcusson A., von Bulow V., Schuster N., Zhang S., Heldin C.H., Landstrom M. Nat. Cell Biol. 10:1199-1207(2008)

3. "NUMBL interacts with TRAF6 and promotes the degradation of TRAF6." Zhou L., Ma Q., Shi H., Huo K. Biochem. Biophys. Res. Commun. 392:409-414(2010)

Sequence Similarity

Belongs to the TNF receptor-associated factor family. A subfamily.

Tissue Specificity

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Post-translational Modification

Sumoylated on Lys-124, Lys-142 and Lys-453 with SUMO1.; Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. Deubiquitinated by USP10 in a TANK-dependent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage. LRRC19 induces 'Lys-63' ubiquitination.

Subcellular Location

Cytoplasm, nucleus

UNIPROT

SwissProt: Q9Y4K3 Human

SwissProt: P70196 Mouse

SwissProt: B5DF45 Rat

Synonyms

E3 ubiquitin-protein ligase TRAF6 antibody

Interleukin 1 signal transducer antibody

Interleukin-1 signal transducer antibody

MGC 3310 antibody

MGC:3310 antibody

MGC3310 antibody

OTTHUMP00000232772 antibody

OTTHUMP00000232773 antibody

RING finger protein 85 antibody

RNF 85 antibody

Expand

Images

  • Western blot analysis of TRAF6 on different lysates with Rabbit anti-TRAF6 antibody (<a href="/products/R1311-2" style="font-weight: bold;text-decoration: underline;">R1311-2</a>) at 1/1,000 dilution.<br /><br />Lane 1: CTLL-2 (Mouse T lymphocyte) cell lysate<br />Lane 2: EL4 (Mouse lymphoma cell) cell lysate<br />Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate<br />Lane 4: L6 (Rat skeletal muscle myoblast) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 120 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1311-2" style="font-weight: bold;text-decoration: underline;">R1311-2</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 59.6 kDa<br />Observed band size: 60 kDa

    Western blot analysis of TRAF6 on different lysates with Rabbit anti-TRAF6 antibody (R1311-2) at 1/1,000 dilution.

    Lane 1: CTLL-2 (Mouse T lymphocyte) cell lysate
    Lane 2: EL4 (Mouse lymphoma cell) cell lysate
    Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
    Lane 4: L6 (Rat skeletal muscle myoblast) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 120 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1311-2, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 59.6 kDa
    Observed band size: 60 kDa

  • Western blot analysis of TRAF6 on different lysates with Rabbit anti-TRAF6 antibody (<a href="/products/R1311-2" style="font-weight: bold;text-decoration: underline;">R1311-2</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 30 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1311-2" style="font-weight: bold;text-decoration: underline;">R1311-2</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 59.6 kDa<br />Observed band size: 60 kDa

    Western blot analysis of TRAF6 on different lysates with Rabbit anti-TRAF6 antibody (R1311-2) at 1/1,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 30 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1311-2, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 59.6 kDa
    Observed band size: 60 kDa

  • ICC staining TRAF6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TRAF6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Western blot analysis of TRAF6 on hybrid fish (crucian-carp) heart tissue lysate using anti-TRAF6 antibody at 1/500 dilution.

    Western blot analysis of TRAF6 on hybrid fish (crucian-carp) heart tissue lysate using anti-TRAF6 antibody at 1/500 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated