T-bet / Tbx21 Rabbit Polyclonal Antibody
Catalog# ER1802-65
T-bet / Tbx21 Rabbit Polyclonal Antibody
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WB
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FC
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IHC-P
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Human
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unconjugated
Overview
Product Name
T-bet / Tbx21 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human Tbx21 aa 486-535 / 535.
Species Reactivity
Human
Validated Applications
WB, FC, IHC-P
Molecular Weight
Predicted band size: 58 kDa
Positive Control
NK-92 cell lysates, Raji, human lymph nodes tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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FC
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1ug/mL
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IHC-P
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1:500
Target
Function
T-box transcription factor TBX21, also called T-bet (T-box expressed in T cells), is a protein that in humans is encoded by the TBX21 gene. Though being for long thought of only as a master regulator of type 1 immune response, T-bet has recently been shown to be implicated in development of various immune cell subsets and maintenance of mucosal homeostasis. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNg). Expression of the human ortholog also correlates with IFNg expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Background References
1. Yang X et al. TBX21 inhibits colorectal cancer metastasis through ARHGAP29/GSK3beta inhibitory signaling- and MYCT1/ZO-1 signaling-dependent manner. Int J Biol Sci. 2025 Jan
2. Zou Z et al. TBX21 knockdown attenuates neuroinflammation induced by intracerebral hemorrhage via the SIRT1-WDR5-H3K4me3 axis. Brain Res Bull. 2025 Sep
Tissue Specificity
T-cell specific. Expressed in regulatory T (TReg) cells.
Post-translational Modification
Phosphorylations at Ser-52, Tyr-76, Ser-224 and Ser-508 are regulated by mTORC1. Phosphorylation at Tyr-525 is essential for its interaction GATA3. Phosphorylation at Tyr-219, Tyr-265 and Tyr-304 enhances its transcriptional activator activity. Phosphorylation at Thr-302 is required for its interaction with NFATC2.; Ubiquitinated at Lys-313, leading to its degradation by the proteasome. Ubiquitination is essential for controlling protein stability, binding to the T-box-binding element of the IFN-gamma promoter, and for interaction with NFATC2 through induction of phosphorylation at Thr-302. Deubiquitinated by USP10 leading to its stabilization (By similarity).
Subcellular Location
Nucleus.
UNIPROT
Synonyms
T bet antibody
T box 21 antibody
T box expressed in T cells antibody
T box protein 21 antibody
T box transcription factor TBX21 antibody
T cell specific T box transcription factor antibody
T cell specific T box transcription factor T bet antibody
T PET antibody
T-box protein 21 antibody
T-box transcription factor TBX21 antibody
ExpandImages
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Western blot analysis of T-bet / Tbx21 on NK-92 cell lysates with Rabbit anti-T-bet / Tbx21 antibody (ER1802-65) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 65 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-65) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of Raji cells labeling T-bet / Tbx21.
Cells were fixed and permeabilized. Then stained with the primary antibody (ER1802-65, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-T-bet / Tbx21 antibody (ER1802-65) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-65) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"