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SOX2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1106-1

SOX2 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SOX2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within N-terminal human SOX2.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 34 kDa

Positive Control

MCF7 cell lysate, F9 cell lysate, C6 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

RRID

AB_3073179

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

Target

Function

SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential to maintain self-renewal of undifferentiated embryonic stem cells. Sox2, Oct-4 and Nanog co-operate in this network, and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency. POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells

Background References

1. "Mutations in SOX2 cause anophthalmia."Fantes J., Ragge N.K., Lynch S.-A., McGill N.I., Collin J.R.O., Howard-Peebles P.N., Hayward C., Vivian A.J., Williamson K., van Heyningen V., FitzPatrick D.R.Nat. Genet. 33:461-463(2003)

2. "Induction of pluripotent stem cells from adult human fibroblasts by defined factors."Takahashi K., Tanabe K., Ohnuki M., Narita M., Ichisaka T., Tomoda K., Yamanaka S.Cell 131:861-872(2007)

Post-translational Modification

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P48431 Human

SwissProt: P48432 Mouse

Entrez Gene: 499593 Rat

Synonyms

ANOP3 antibody

cb236 antibody

Delta EF2a antibody

lcc antibody

MCOPS3 antibody

MGC148683 antibody

MGC2413 antibody

RGD1565646 antibody

Sex determining region Y box 2 antibody

SOX 2 antibody

Expand

Images

  • Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (<a href="/products/R1106-1" style="font-weight: bold;text-decoration: underline;">R1106-1</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate (20 µg/Lane)<br />Lane 2: F9 cell lysate (20 µg/Lane)<br />Lane 3: C6 cell lysate (20 µg/Lane)<br />Lane 4: Human brain tissue lysate (40 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 6: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 34 kDa<br />Observed band size: 34 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1106-1" style="font-weight: bold;text-decoration: underline;">R1106-1</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (R1106-1) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate (20 µg/Lane)
    Lane 2: F9 cell lysate (20 µg/Lane)
    Lane 3: C6 cell lysate (20 µg/Lane)
    Lane 4: Human brain tissue lysate (40 µg/Lane)
    Lane 5: Mouse brain tissue lysate (40 µg/Lane)
    Lane 6: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1106-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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