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Transglutaminase 2 Recombinant Rabbit Monoclonal Antibody [JU30-02]

Usd: 385

Specification

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Catalog# ET1706-35

Transglutaminase 2 Recombinant Rabbit Monoclonal Antibody [JU30-02]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Transglutaminase 2 Recombinant Rabbit Monoclonal Antibody [JU30-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Transglutaminase 2 aa 578-627 / 687.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 77 kDa

Positive Control

K-562 cell lysate, HeLa cell lysate, HUVEC cell lysate, Mouse placenta tissue lysate, human placenta tissue, mouse placenta tissue, rat placenta tissue.

Conjugation

unconjugated

Clone Number

JU30-02

RRID

AB_3070656

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:200

Target

Function

Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein.

Background References

1. Espitia Pinzon N et al. Astrocyte-derived tissue Transglutaminase affects fibronectin deposition, but not aggregation, during cuprizone-induced demyelination. Sci Rep 7:40995 (2017).

2. Merlino G et al. Stromal Activation by Tumor Cells: An in Vitro Study in Breast Cancer. Microarrays (Basel) 5:2 (2016).

Sequence Similarity

Belongs to the transglutaminase superfamily. Transglutaminase family.

Subcellular Location

Cytosol. Plasma Membrane.

UNIPROT

SwissProt: P21980 Human

SwissProt: P21981 Mouse

Entrez Gene: 56083 Rat

Synonyms

ALPHA SUBUNIT antibody

C polypeptide antibody

EC 2.3.2.13 antibodyepididymis secretory protein Li 45 antibody

G alpha h antibody

G[a]h antibody

Gh CLASS G ALPHA h antibody

GNAH antibody

GNAH G PROTEIN antibody

H POLYPEPTIDE antibody

HEL-S-45 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody [JU30-02] (<a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a>) at 1:1,000 dilution.<br /><br />Lane 1: Wild-type MDCK whole cell lysate.<br />Lane 2: TGM2 knockout MDCK whole cell lysate.<br /><br /><a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a> was shown to specifically react with Transglutaminase 2 in wild-type Hela cells. No band was observed when TGM2 knockout samples were tested. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Transglutaminase 2 antibody (<a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a>, 1/1,000) and Anti-HSP90 antibody (<a href="/products/ET1605-56" style="font-weight: bold;text-decoration: underline;">ET1605-56</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><br />Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

    ☑ Knockout (KO)

    All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody [JU30-02] (ET1706-35) at 1:1,000 dilution.

    Lane 1: Wild-type MDCK whole cell lysate.
    Lane 2: TGM2 knockout MDCK whole cell lysate.

    ET1706-35 was shown to specifically react with Transglutaminase 2 in wild-type Hela cells. No band was observed when TGM2 knockout samples were tested. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Transglutaminase 2 antibody (ET1706-35, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

  • Western blot analysis of Transglutaminase 2 on different lysates with Rabbit anti-Transglutaminase 2 antibody (<a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: HUVEC cell lysate<br />Lane 4: Mouse placenta tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 77 kDa<br />Observed band size: 70-77 kDa<br /><br />Exposure time: 12 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Transglutaminase 2 on different lysates with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: HUVEC cell lysate
    Lane 4: Mouse placenta tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 77 kDa
    Observed band size: 70-77 kDa

    Exposure time: 12 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-35) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Transglutaminase 2 antibody (<a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-35) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Transglutaminase 2 antibody (<a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-35" style="font-weight: bold;text-decoration: underline;">ET1706-35</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-35) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Transglutaminase 2 Rabbit Polyclonal Antibody

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat,Dog

Conjugate: unconjugated