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TNPO3 Recombinant Rabbit Monoclonal Antibody [JG38-77]

Usd: 385

Specification

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Catalog# ET7108-80

TNPO3 Recombinant Rabbit Monoclonal Antibody [JG38-77]

Application

  • WB

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TNPO3 Recombinant Rabbit Monoclonal Antibody [JG38-77]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TNPO3 aa 830-923 / 923.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC, IF-Tissue

Molecular Weight

Predicted band size: 104 kDa

Positive Control

K-562 cell lysate, HL-60 cell lysate, HeLa cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate, mouse kidney tissue, rat kidney tissue, human small intestine tissue, K-562.

Conjugation

unconjugated

Clone Number

JG38-77

RRID

AB_3070874

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:1,000

  • IF-Tissue

  • 1:50

Target

Function

The Importin complex consists of Importin-α and Importin-β proteins which assist in transport of arginine- or serine-rich (SR) proteins across the nucleus. Importin-12, also known as Transportin-3 or Transportin-SR, is a member of the Importin-β family and functions as a nuclear transport receptor for serine/arginine-rich proteins. Through recognition of phosphorylated RS domains, Importin-12 mediates the nuclear import of several SR proteins, such as splicing factors SFRS1 and SFRS2. By regulating the nucleocytoplasmic transport of these and other SR mRNA splicing factors, Importin-12 controls their access to mRNA and, therefore, acts a transcriptional regulator.

Background References

1. Kataoka N et al. Transportin-SR, a nuclear import receptor for SR proteins. J Cell Biol 145:1145-1152 (1999).

2. Lai M-C et al. A human importin-beta family protein, transportin-SR2, interacts with the phosphorylated RS domain of SR proteins. J Biol Chem 275:7950-7957 (2000).

Tissue Specificity

Expressed in skeletal muscle.

Subcellular Location

Nucleus. Cytoplasm.

UNIPROT

SwissProt: Q9Y5L0 Human

SwissProt: Q6P2B1 Mouse

Entrez Gene: 296954 Rat

Synonyms

TNPO 3 antibody

Imp12 antibody

Importin 12 antibody

Importin-12 antibody

IPO12 antibody

MTR10A antibody

TNPO3 antibody

TNPO3_HUMAN antibody

Transportin 3 antibody

Transportin SR antibody

Expand

Images

  • Western blot analysis of TNPO3 on different lysates with Rabbit anti-TNPO3 antibody (<a href="/products/ET7108-80" style="font-weight: bold;text-decoration: underline;">ET7108-80</a>) at 1/2,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: HL-60 cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: Ramos cell lysate (20 µg/Lane)<br />Lane 5: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 6: Mouse pancreas tissue lysate (40 µg/Lane)<br />Lane 7: Rat pancreas tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 104 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-80" style="font-weight: bold;text-decoration: underline;">ET7108-80</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TNPO3 on different lysates with Rabbit anti-TNPO3 antibody (ET7108-80) at 1/2,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: HL-60 cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: Ramos cell lysate (20 µg/Lane)
    Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 6: Mouse pancreas tissue lysate (40 µg/Lane)
    Lane 7: Rat pancreas tissue lysate (40 µg/Lane)

    Predicted band size: 104 kDa
    Observed band size: 100 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-80) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TNPO3 antibody (<a href="/products/ET7108-80" style="font-weight: bold;text-decoration: underline;">ET7108-80</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-80" style="font-weight: bold;text-decoration: underline;">ET7108-80</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TNPO3 antibody (ET7108-80) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TNPO3 antibody (<a href="/products/ET7108-80" style="font-weight: bold;text-decoration: underline;">ET7108-80</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-80" style="font-weight: bold;text-decoration: underline;">ET7108-80</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TNPO3 antibody (ET7108-80) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of K-562 cells labeling TNPO3.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET7108-80" style="font-weight: bold;text-decoration: underline;">ET7108-80</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of K-562 cells labeling TNPO3.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-80, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation