SMC3 Recombinant Rabbit Monoclonal Antibody [JM10-75]
Catalog# ET1703-35
SMC3 Recombinant Rabbit Monoclonal Antibody [JM10-75]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
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Zebrafish
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unconjugated
Overview
Product Name
SMC3 Recombinant Rabbit Monoclonal Antibody [JM10-75]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SMC3 aa 1994-1217 / 1217.
Species Reactivity
Human, Mouse, Rat, Zebrafish
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Molecular Weight
Predicted band size: 142 kDa
Positive Control
HepG2 cell lysate, NIH-3T3 cell lysate, PC12 cell lysate, Hela, HepG2, NIH-3T3, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse colon tissue, zebrafish tissue.
Conjugation
unconjugated
Clone Number
JM10-75
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology.
Background References
1. Kracker S et al. An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex. J Allergy Clin Immunol 135:998-1007.e6 (2015).
2. Qiu Z et al. Functional interactions between NURF and Ctcf regulate gene expression. Mol Cell Biol 35:224-37 (2015).
Sequence Similarity
Belongs to the SMC family. SMC3 subfamily.
Post-translational Modification
Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation.; Phosphorylated at Ser-1083 in a SPO11-dependent manner.; Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.
Subcellular Location
Centromere, Chromosome, Nucleus.
Synonyms
BAM antibody
Bamacan antibody
Basement membrane associated chondroitin proteoglycan antibody
Basement membrane-associated chondroitin proteoglycan antibody
BMH antibody
CDLS3 antibody
chondroitin sulfate proteoglycan 6 (bamacan) antibody
Chondroitin sulfate proteoglycan 6 antibody
Chromosome associated polypeptide antibody
Chromosome-associated polypeptide antibody
ExpandImages
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Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution.
Positive control:
Line1: HepG2 cell lysate
Line2: NIH-3T3 cell lysate
Line3:PC12 cell lysate -
ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"