Skip to content

SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [JE56-93]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA720005

SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [JE56-93]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [JE56-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SLC31A1 aa 1-82.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 21 kDa

Positive Control

A549 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse liver tissue lysate, Rat brain tissue lysate, HeLa cell lysate, Caco-2 cell lysate, C2C12 cell lysate.

Conjugation

unconjugated

Clone Number

JE56-93

RRID

AB_3071946

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

High affinity copper uptake protein 1 (Ctr1) is a protein that in humans is encoded by the SLC31A1 gene. Copper is an element essential for life, but excessive copper can be toxic or even lethal to the cell. Therefore, cells have developed sophisticated ways to maintain a critical copper balance, with the intake, export, and intracellular compartmentalization or buffering of copper strictly regulated. The 2 related genes ATP7A (MIM 300011) and ATP7B (MIM 606882), responsible for the human diseases Menkes syndrome (MIM 309400) and Wilson disease (MIM 277900), respectively, are involved in copper export. In S. cerevisiae, the copper uptake genes CTR1, CTR2, and CTR3 have been identified, and in human the CTR1 and CTR2 (MIM 603088) genes have been identified.

Background References

1. Yu Z. et. al. Blockage of SLC31A1-dependent copper absorption increases pancreatic cancer cell autophagy to resist cell death. Cell Prolif. 2019 Mar

2. Sun C. et. al. Genetic polymorphism of SLC31A1 is associated with clinical outcomes of platinum-based chemotherapy in non-small-cell lung cancer patients through modulating microRNA-mediated regulation. Oncotarget. 2018 May

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: O15431 Human

SwissProt: Q8K211 Mouse

SwissProt: Q9JK41 Rat

Synonyms

Copper transport 1 homolog antibody

Copper transporter 1 antibody

COPT1 antibody

COPT1_HUMAN antibody

CTR1 antibody

hCTR1 antibody

High affinity copper uptake protein 1 antibody

SLC31A1 antibody

solute carrier family 31 (copper tansporters) member 1 antibody

Solute carrier family 31 member 1 antibody

Images

  • Western blot analysis of SLC31A1 / CTR1 on different lysates with Rabbit anti-SLC31A1 / CTR1 antibody (<a href="/products/HA720005" style="font-weight: bold;text-decoration: underline;">HA720005</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 3: C6 cell lysate (20 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 5: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 21 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720005" style="font-weight: bold;text-decoration: underline;">HA720005</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SLC31A1 / CTR1 on different lysates with Rabbit anti-SLC31A1 / CTR1 antibody (HA720005) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: Neuro-2a cell lysate (20 µg/Lane)
    Lane 3: C6 cell lysate (20 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)
    Lane 5: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 21 kDa
    Observed band size: 30 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720005) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of SLC31A1 / CTR1 on different lysates with Rabbit anti-SLC31A1 / CTR1 antibody (<a href="/products/HA720005" style="font-weight: bold;text-decoration: underline;">HA720005</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Caco-2 cell lysate<br />Lane 3: Neuro-2a cell lysate<br />Lane 4: C2C12 cell lysate<br />Lane 5: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720005" style="font-weight: bold;text-decoration: underline;">HA720005</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SLC31A1 / CTR1 on different lysates with Rabbit anti-SLC31A1 / CTR1 antibody (HA720005) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Caco-2 cell lysate
    Lane 3: Neuro-2a cell lysate
    Lane 4: C2C12 cell lysate
    Lane 5: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 30 kDa

    Exposure time: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720005) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation