SFPQ Recombinant Rabbit Monoclonal Antibody [JU33-99]
Catalog# ET7107-02
SFPQ Recombinant Rabbit Monoclonal Antibody [JU33-99]
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WB
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IHC-P
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SFPQ Recombinant Rabbit Monoclonal Antibody [JU33-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SFPQ aa 658-707 / 707.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell, IF-Tissue
Molecular Weight
Predicted band size: 76 kDa
Positive Control
Jurkat cell lysate, 293T cell lysate, HeLa cell lysate, K-562 cell lysate, Daudi cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Mouse lung tissue lysate, HeLa, Daudi, mouse brain tissue, rat brain tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse testis tissue.
Conjugation
unconjugated
Clone Number
JU33-99
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:1,000
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IF-Cell
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1:500
Target
Function
DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation.
Background References
1. Patton J G et al. Cloning and characterization of PSF, a novel pre-mRNA splicing factor. Genes Dev 7:393-406 (1993).
2. Lukong K E et al. BRK phosphorylates PSF promoting its cytoplasmic localization and cell cycle arrest. Cell Signal 21:1415-1422 (2009).
Post-translational Modification
The N-terminus is blocked.; Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA. Phosphorylation of C-terminal tyrosines promotes its cytoplasmic localization, impaired its binding to polypyrimidine RNA and led to cell cycle arrest. In resting T-cells is phosphorylated at Thr-687 by GSK3B which is proposed to promote association with THRAP and to prevent binding to PTPRC/CD45 pre-mRNA; T-cell activation leads to reduced phosphorylation at Thr-687.
Subcellular Location
Nucleus. Cytoplasm.
Synonyms
100 kDa DNA pairing protein antibody
100 kDa DNA-pairing protein antibody
100 kDa subunit antibody
DNA binding p52/p100 complex 100 kDa subunit antibody
DNA-binding p52/p100 complex antibody
hPOMp100 antibody
Polypyrimidine tract binding protein associated splicing factor antibody
Polypyrimidine tract-binding protein-associated-splicing factor antibody
POMP100 antibody
PPP1R140 antibody
ExpandImages
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Western blot analysis of SFPQ on different lysates with Rabbit anti-SFPQ antibody (ET7107-02) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Daudi cell lysate (20 µg/Lane)
Lane 6: Neuro-2a cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: C2C12 cell lysate (20 µg/Lane)
Lane 9: C6 cell lysate (20 µg/Lane)
Lane 10: Mouse testis tissue lysate (40 µg/Lane)
Lane 11: Mouse lung tissue lysate (40 µg/Lane)
Predicted band size: 76 kDa
Observed band size: 100 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-02) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling SFPQ with Rabbit anti-SFPQ antibody (ET7107-02) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SFPQ antibody (ET7107-02) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of Daudi cells labeling SFPQ with Rabbit anti-SFPQ antibody (ET7107-02) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SFPQ antibody (ET7107-02) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SFPQ antibody (ET7107-02) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-02) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SFPQ antibody (ET7107-02) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-02) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"