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SCF Recombinant Rabbit Monoclonal Antibody [SC61-06]

Usd: 385

Specification

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Catalog# ET1610-77

SCF Recombinant Rabbit Monoclonal Antibody [SC61-06]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SCF Recombinant Rabbit Monoclonal Antibody [SC61-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SCF aa 36-85 / 273.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 31 kDa

Positive Control

Raji cell lysate, COLO205 cell lysate, HT-29 cell lysate, A549 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human prostate cancer tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

SC61-06

RRID

AB_3069962

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:1,000

Target

Function

Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger form of SCF is expressed in fibroblasts, brain and thymus, while the smaller form is found in spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells.

Background References

1. Xiang FL et al. Cardiac-specific overexpression of human stem cell factor promotes epicardial activation and arteriogenesis after myocardial infarction. Circ Heart Fail 7:831-42 (2014).

2. Nishikori Y et al. The role of mast cells in cutaneous wound healing in streptozotocin-induced diabetic mice. Arch Dermatol Res 306:823-35 (2014).

Sequence Similarity

Belongs to the SCF family.

Post-translational Modification

A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.; Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.; A soluble form exists as a cleavage product of the extracellular domain.

Subcellular Location

Cell membrane, Cytoplasm, Secreted.

UNIPROT

SwissProt: P21583 Human

SwissProt: P20826 Mouse

SwissProt: P21581 Rat

Synonyms

C kit ligand antibody

C-kit ligand antibody

Ckit ligand antibody

DCUA antibody

DFNA69 antibody

DKFZp686F2250 antibody

familial progressive hyperpigmentation 2 antibody

FPH2 antibody

FPHH antibody

KIT ligand antibody

Expand

Images

  • Western blot analysis of SCF on different lysates with Rabbit anti-SCF antibody (<a href="/products/ET1610-77" style="font-weight: bold;text-decoration: underline;">ET1610-77</a>) at 1/2,000 dilution.<br /><br />Lane 1: Raji cell lysate (20 µg/Lane)<br />Lane 2: COLO205 cell lysate (20 µg/Lane)<br />Lane 3: HT-29 cell lysate (20 µg/Lane)<br />Lane 4: A549 cell lysate (20 µg/Lane)<br />Lane 5: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 6: Rat kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 31 kDa<br />Observed band size: 35 kDa<br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-77" style="font-weight: bold;text-decoration: underline;">ET1610-77</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SCF on different lysates with Rabbit anti-SCF antibody (ET1610-77) at 1/2,000 dilution.

    Lane 1: Raji cell lysate (20 µg/Lane)
    Lane 2: COLO205 cell lysate (20 µg/Lane)
    Lane 3: HT-29 cell lysate (20 µg/Lane)
    Lane 4: A549 cell lysate (20 µg/Lane)
    Lane 5: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 6: Rat kidney tissue lysate (40 µg/Lane)

    Predicted band size: 31 kDa
    Observed band size: 35 kDa
    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-77) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-SCF antibody (<a href="/products/ET1610-77" style="font-weight: bold;text-decoration: underline;">ET1610-77</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-77" style="font-weight: bold;text-decoration: underline;">ET1610-77</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-SCF antibody (ET1610-77) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SCF antibody (<a href="/products/ET1610-77" style="font-weight: bold;text-decoration: underline;">ET1610-77</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-77" style="font-weight: bold;text-decoration: underline;">ET1610-77</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SCF antibody (ET1610-77) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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Application: WB,IP,IF,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated