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SAE1 Recombinant Rabbit Monoclonal Antibody [JG35-88]

Usd: 385

Specification

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Catalog# ET7108-22

SAE1 Recombinant Rabbit Monoclonal Antibody [JG35-88]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SAE1 Recombinant Rabbit Monoclonal Antibody [JG35-88]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SAE1 aa 201-249 / 346.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 38 kDa

Positive Control

Jurkat cell lysate, HeLa cell lysate, 293T cell lysate, human colon cancer tissue, human skin tissue.

Conjugation

unconjugated

Clone Number

JG35-88

RRID

AB_3070822

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Proteolytic degradation by the ubiquitin (Ub) system is essential for normal cell cycle progression, cellular differentiation and stress responses. Proteins conjugated to Ub are marked for progressive degradation by the 26S Proteasome. AOS-1, also designated SUMO-1-activating enzyme or ubiquitin-like 1-activating enzyme E1A, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the UBL1 conjugation pathway. AOS-1, which is a dimeric enzyme, functions as a UBLI E1 ligase, mediating the ATP-dependent activation of UBL1. AOS-1 can bind with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1.

Background References

1. Okuma T et al. In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2. Biochem Biophys Res Commun 254:693-698 (1999).

2. Gong L et al. Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett 448:185-189 (1999).

Sequence Similarity

Belongs to the ubiquitin-activating E1 family.

Tissue Specificity

Expression level increases during S phase and drops in G2 phase (at protein level).

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9UBE0 Human

Synonyms

Activator of SUMO1 antibody

AOS1 antibody

HSPC140 antibody

Sae1 antibody

SAE1_HUMAN antibody

Sentrin/SUMO activating protein AOS1 antibody

SUA1 antibody

SUMO 1 activating enzyme E1 N subunit antibody

SUMO 1 activating enzyme subunit 1 antibody

SUMO-activating enzyme subunit 1 antibody

Expand

Images

  • Western blot analysis of SAE1 on different lysates with Rabbit anti-SAE1 antibody (<a href="/products/ET7108-22" style="font-weight: bold;text-decoration: underline;">ET7108-22</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: 293T cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-22" style="font-weight: bold;text-decoration: underline;">ET7108-22</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SAE1 on different lysates with Rabbit anti-SAE1 antibody (ET7108-22) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: 293T cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 14 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SAE1 antibody (<a href="/products/ET7108-22" style="font-weight: bold;text-decoration: underline;">ET7108-22</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-22" style="font-weight: bold;text-decoration: underline;">ET7108-22</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SAE1 antibody (ET7108-22) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-22) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-SAE1 antibody (<a href="/products/ET7108-22" style="font-weight: bold;text-decoration: underline;">ET7108-22</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-22" style="font-weight: bold;text-decoration: underline;">ET7108-22</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-SAE1 antibody (ET7108-22) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-22) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation