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Prostate Specific Antigen Recombinant Rabbit Monoclonal Antibody [SD07-45]

Usd: 385

Specification

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Catalog# ET1612-19

Prostate Specific Antigen Recombinant Rabbit Monoclonal Antibody [SD07-45]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Prostate Specific Antigen Recombinant Rabbit Monoclonal Antibody [SD07-45]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Prostate Specific Antigen aa 198-261/261.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 29 kDa

Positive Control

PC-3M, human prostate tissue, human prostate carcinoma tissue.

Conjugation

unconjugated

Clone Number

SD07-45

RRID

AB_3070091

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:500

Target

Function

Prostate specific antigen (PSA), also designated γ-seminoprotein, seminin, p30 antigen, semenogelase, and kallikrein 3 (KLK3), was first identified as a glycoprotein in human seminal plasma. PSA was determined by sequence similarity to be a member of the kallikrein subfamily of trypsin proteases. PSA is a serine protease that hydrolyzes the major human seminal protein, the seminal plasma mobility inhibitor precursor, or semenogelin I (SPMIP or SgI), which leads to semen liquification. PSA production and expression are highest in normal, benign hyperplastic and cancerous tissues of the prostate, although PSA has also been detected in accessory male sex glands and in breast cancer. PSA has been identified as an aid in the early detection of prostate cancer and is a commonly used tumor marker.

Background References

1. Garg S et al. Point-of-Care Prostate Specific Antigen Testing: Examining Translational Progress toward Clinical Implementation. ACS Sens. 2023 Oct

2. Dowlatshahi S et al. Electrochemical prostate-specific antigen biosensors based on electroconductive nanomaterials and polymers. Clin Chim Acta. 2021 May

Sequence Similarity

Belongs to the peptidase S1 family. Kallikrein subfamily.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P07288 Human

Synonyms

antigen, prostate-specific antibody

APS antibody

Gamma seminoprotein antibody

Gamma-seminoprotein antibody

hK3 antibody

Kallikrein 3 antibody

Kallikrein related peptidase 3 antibody

Kallikrein-3 antibody

KLK 3 antibody

KLK2A1 antibody

Expand

Images

  • ICC staining of Prostate Specific Antigen in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1612-19" style="font-weight: bold;text-decoration: underline;">ET1612-19</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Prostate Specific Antigen in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Prostate Specific Antigen antibody (<a href="/products/ET1612-19" style="font-weight: bold;text-decoration: underline;">ET1612-19</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-19" style="font-weight: bold;text-decoration: underline;">ET1612-19</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Prostate Specific Antigen antibody (ET1612-19) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Prostate Specific Antigen antibody (<a href="/products/ET1612-19" style="font-weight: bold;text-decoration: underline;">ET1612-19</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-19" style="font-weight: bold;text-decoration: underline;">ET1612-19</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Prostate Specific Antigen antibody (ET1612-19) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-19) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: prostate <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Human

    Site: prostate

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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