Prealbumin Recombinant Rabbit Monoclonal Antibody [JF1016]
Catalog# ET1702-42
Prealbumin Recombinant Rabbit Monoclonal Antibody [JF1016]
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WB
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IHC-P
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IP
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Human
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unconjugated
Overview
Product Name
Prealbumin Recombinant Rabbit Monoclonal Antibody [JF1016]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Prealbumin aa 11-50 / 147.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 16 kDa
Positive Control
Human lung tissue lysates, human liver tissue, human pancreas tissue.
Conjugation
unconjugated
Clone Number
JF1016
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:50-1:200
Target
Function
Transthyretin (TTR or TBPA) is a transport protein in the plasma and cerebrospinal fluid that transports the thyroid hormone thyroxine (T4) and retinol to the liver. This is how transthyretin gained its name: transports thyroxine and retinol. The liver secretes TTR into the blood, and the choroid plexus secretes TTR into the cerebrospinal fluid. TTR was originally called prealbumin (or thyroxine-binding prealbumin) because it migrated faster than albumin on electrophoresis gels. Prealbumin was felt to be a misleading name, it is not a synthetic precursor of albumin. The alternative name TTR was proposed by DeWitt Goodman in 1981. Human transthyretrin protein is encoded by the TTR gene, which is located on the long arm of chromosome 18, in cytogenetic band 18q12.1.
Background References
1. Ranasinghe RN et al. Prealbumin: The clinical utility and analytical methodologies. Ann Clin Biochem. 2022 Jan
2. Yu Q et al. Serum prealbumin as a predictor of adverse outcomes in patients with heart failure: a systematic review and meta-analysis. Biomark Med. 2022 May
Sequence Similarity
Belongs to the transthyretin family.
Tissue Specificity
Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
Post-translational Modification
Not glycosylated under normal conditions. Following unfolding, caused for example by variant AMYL-TTR 'Gly-38', the cryptic Asn-118 site is exposed and glycosylated by STT3B-containing OST complex, leading to its degradation by the ER-associated degradation (ERAD) pathway.
Subcellular Location
Secreted, Cytoplasm.
UNIPROT
Synonyms
Amyloid polyneuropathy antibody
Amyloidosis I antibody
ATTR antibody
Carpal tunnel syndrome 1 antibody
CTS antibody
CTS1 antibody
Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
Dystransthyretinemic hyperthyroxinemia antibody
Epididymis luminal protein 111 antibody
HEL111 antibody
ExpandImages
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Western blot analysis of Prealbumin on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Prealbumin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Prealbumin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-42, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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