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Phospho-Synapsin I (S9) Recombinant Rabbit Monoclonal Antibody [SN63-04]

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Specification

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Catalog# ET1611-26

Phospho-Synapsin I (S9) Recombinant Rabbit Monoclonal Antibody [SN63-04]

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

Predicted reactivity

  • Human

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-Synapsin I (S9) Recombinant Rabbit Monoclonal Antibody [SN63-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser9 of Human Synapsin I aa 1-50 / 705.

Species Reactivity

Mouse, Rat (Predicted: Human)

Predicted species support after-sales service

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 74 kDa

Positive Control

Mouse brain tissue lysates, rat brain tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

SN63-04

RRID

AB_3070003

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

Target

Function

Synapsin I, is the collective name for Synapsin Ia and Synapsin Ib, two nearly identical phosphoproteins that in humans are encoded by the SYN1 gene. In its phosphorylated form, Synapsin I may also be referred to as phosphosynaspin I. Synapsin I is the first of the proteins in the synapsin family of phosphoproteins in the synaptic vesicles present in the central and peripheral nervous systems. Synapsin Ia and Ib are close in length and almost the same in make up, however, Synapsin Ib stops short of the last segment of the C-terminal in the amino acid sequence found in Synapsin Ia.

Background References

1. Van Boxstael E et al. Treating seizures in SYN1-related epilepsy: a systematic review. Seizure. 2025 Sep

2. Xiong J et al. Familial SYN1 variants related neurodevelopmental disorders in Asian pediatric patients. BMC Med Genomics. 2021 Jul

Sequence Similarity

Belongs to the synapsin family.

Post-translational Modification

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal.; Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.

Subcellular Location

Synapse, Golgi apparatus, Presynapse, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.

UNIPROT

SwissProt: P17600 Human

SwissProt: O88935 Mouse

SwissProt: P09951 Rat

Synonyms

Brain protein 4.1 antibody

SYN 1 antibody

SYN 1a antibody

SYN 1b antibody

SYN I antibody

SYN1 antibody

SYN1_HUMAN antibody

SYN1a antibody

SYN1b antibody

Synapsin 1 antibody

Expand

Images

  • Western blot analysis of Phospho-Synapsin I (S9) on mouse brain tissue lysates with Rabbit anti-Phospho-Synapsin I (S9) antibody (<a href="/products/ET1611-26" style="font-weight: bold;text-decoration: underline;">ET1611-26</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 77 kDa<br /><br />Exposure time: 30 seconds;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-26" style="font-weight: bold;text-decoration: underline;">ET1611-26</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospho-Synapsin I (S9) on mouse brain tissue lysates with Rabbit anti-Phospho-Synapsin I (S9) antibody (ET1611-26) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 77 kDa

    Exposure time: 30 seconds;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-26) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-Synapsin I (S9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-26" style="font-weight: bold;text-decoration: underline;">ET1611-26</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-Synapsin I (S9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Synapsin I (S9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-26" style="font-weight: bold;text-decoration: underline;">ET1611-26</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Synapsin I (S9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation