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Phospho-GluR1 (S845) Recombinant Rabbit Monoclonal Antibody [JJ2009]

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Specification

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Catalog# ET1701-28

Phospho-GluR1 (S845) Recombinant Rabbit Monoclonal Antibody [JJ2009]

Application

  • WB

  • FC

Reactivity

  • Mouse

  • Rat

Predicted reactivity

  • Human

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-GluR1 (S845) Recombinant Rabbit Monoclonal Antibody [JJ2009]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser845 of Human GluR1 aa 831-880 / 906.

Species Reactivity

Mouse, Rat (Predicted: Human)

Predicted species support after-sales service

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 102 kDa

Positive Control

Mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate the membrane treated with λpp for 1 hour, rat brain tissue lysate the membrane treated with λpp for 1 hour, N2A.

Conjugation

unconjugated

Clone Number

JJ2009

RRID

AB_3070196

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:10-1:50

Target

Function

Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca2+ ions. The NMDA receptors consist of five subunits: epsilion 1, 2, 3, 4 and one zeta subunit. The zeta subunit is expressed throughout the brainstem whereas the four epsilon subunits display limited distribution.

Background References

1. He XY et al. GluA1 signal peptide determines the spatial assembly of heteromeric AMPA receptors. Proc Natl Acad Sci U S A 113:E5645-54 (2016).

2. Han M & Kim J Effect of dietary iron loading on recognition memory in growing rats. PLoS One 10:e0120609 (2015).

Sequence Similarity

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.

Tissue Specificity

Widely expressed in brain.

Post-translational Modification

Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).; Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2 (By similarity). Phosphorylation of Ser-863 is reduced by induction of long-term depression and increased by induction of long-term potentiation (By similarity).

Subcellular Location

Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection.

UNIPROT

SwissProt: P42261 Human

SwissProt: P23818 Mouse

SwissProt: P19490 Rat

Synonyms

GLUR 1 antibody

GLUR A antibody

AMPA 1 antibody

AMPA selective glutamate receptor 1 antibody

AMPA-selective glutamate receptor 1 antibody

GluA1 antibody

GLUH1 antibody

GluR K1 antibody

GluR-1 antibody

GluR-A antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-GluR1 (S845) on different lysates with Rabbit anti-Phospho-GluR1 (S845) antibody (<a href="/products/ET1701-28" style="font-weight: bold;text-decoration: underline;">ET1701-28</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br />Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour<br />Lane 4: Rat brain tissue lysate, the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 102 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-28" style="font-weight: bold;text-decoration: underline;">ET1701-28</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-GluR1 (S845) on different lysates with Rabbit anti-Phospho-GluR1 (S845) antibody (ET1701-28) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate
    Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour
    Lane 4: Rat brain tissue lysate, the membrane treated with λpp for 1 hour

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of Phospho-GluR1 (S845) was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1701-28" style="font-weight: bold;text-decoration: underline;">ET1701-28</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Phospho-GluR1 (S845) was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-28, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation