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PI 3 Kinase p110 delta Recombinant Rabbit Monoclonal Antibody [JM83-24]

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Specification

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Catalog# ET1705-46

PI 3 Kinase p110 delta Recombinant Rabbit Monoclonal Antibody [JM83-24]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PI 3 Kinase p110 delta Recombinant Rabbit Monoclonal Antibody [JM83-24]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PI 3 Kinase p110 delta aa 197-246 / 1044.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 119 kDa

Positive Control

Raji cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, rat spleen tissue.

Conjugation

unconjugated

Clone Number

JM83-24

RRID

AB_3070573

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform also known as phosphoinositide 3-kinase (PI3K) delta isoform or p110δ is an enzyme that in humans is encoded by the PIK3CD gene. p110δ regulates immune function. In contrast to the other class IA PI3Ks p110α and p110β, p110δ is principally expressed in leukocytes (white blood cells). Genetic and pharmacological inactivation of p110δ has revealed that this enzyme is important for the function of T cells, B cell, mast cells and neutrophils. Hence, p110δ is a promising target for drugs that aim to prevent or treat inflammation, autoimmunity and transplant rejection. Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110α, p110β and p110γ. p110α and p110β interact with SH2/SH3-domain-containing p85 adaptor proteins and with GTP-bound Ras.

Background References

1. Matsukawa K et al. Familial Amyotrophic Lateral Sclerosis-linked Mutations in Profilin 1 Exacerbate TDP-43-induced Degeneration in the Retina of Drosophila melanogaster through an Increase in the Cytoplasmic Localization of TDP-43. J Biol Chem 291:23464-23476 (2016).

2. Guo Z et al. A Legionella effector modulates host cytoskeletal structure by inhibiting actin polymerization. Microbes Infect 16(3):225-36 (2014).

Sequence Similarity

Belongs to the PI3/PI4-kinase family.

Tissue Specificity

Isoform 2 is expressed in normal thymus, lung and spleen tissues, and is detected at low levels in normal lysates from colon and ovarian biopsies, at elevated levels in lysates from colorectal tumors and is abundantly expressed in some ovarian tumors (at protein level). Both isoform 1 and isoform 2 are widely expressed. Isoform 1 is expressed predominantly in leukocytes.

Post-translational Modification

Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O00329 Human

SwissProt: O35904 Mouse

SwissProt: D4A5Q1 Rat

Synonyms

5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody

5-bisphosphate 3-kinase catalytic subunit delta isoform antibody

APDS antibody

GRB1 antibody

IMD14 antibody

p110d antibody

p110delta antibody

p110dp85a antibody

p85-ALPHA antibody

Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody

Expand

Images

  • Western blot analysis of PI 3 Kinase p110 delta on different lysates with Rabbit anti-PI 3 Kinase p110 delta antibody (<a href="/products/ET1705-46" style="font-weight: bold;text-decoration: underline;">ET1705-46</a>) at 1/1,000 dilution.<br /><br />Lane 1: Raji cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 4: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 5: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 119 kDa<br />Observed band size: 105 kDa<br /><br />Exposure time: 12 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-46" style="font-weight: bold;text-decoration: underline;">ET1705-46</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PI 3 Kinase p110 delta on different lysates with Rabbit anti-PI 3 Kinase p110 delta antibody (ET1705-46) at 1/1,000 dilution.

    Lane 1: Raji cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: RAW264.7 cell lysate (20 µg/Lane)
    Lane 4: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 5: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 119 kDa
    Observed band size: 105 kDa

    Exposure time: 12 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-PI 3 Kinase p110 delta antibody (<a href="/products/ET1705-46" style="font-weight: bold;text-decoration: underline;">ET1705-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-46" style="font-weight: bold;text-decoration: underline;">ET1705-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-PI 3 Kinase p110 delta antibody (ET1705-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation