Skip to content

PGC1 beta Recombinant Rabbit Monoclonal Antibody [JM33-24]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1705-65

PGC1 beta Recombinant Rabbit Monoclonal Antibody [JM33-24]

Application

  • WB

  • IP

  • FC

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PGC1 beta Recombinant Rabbit Monoclonal Antibody [JM33-24]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PGC1 beta aa 974-1023 / 1023.

Species Reactivity

Human, Rat

Validated Applications

WB, IP, FC

Molecular Weight

Predicted band size: 113 kDa

Positive Control

SK-Br-3 cell lysate, HeLa cell lysate, HepG2 cell lysate, rat spleen tissue lysates, HL-60.

Conjugation

unconjugated

Clone Number

JM33-24

RRID

AB_3070594

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:200

  • IP

  • 1:10-1:50

Target

Function

Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.

Background References

1. Qi Y et al. PGC-1a Silencing Compounds the Perturbation of Mitochondrial Function Caused by Mutant SOD1 in Skeletal Muscle of ALS Mouse Model. Front Aging Neurosci 7:204 (2015).

2. Kodo K et al. Erythropoietin (EPO) ameliorates obesity and glucose homeostasis by promoting thermogenesis and endocrine function of classical brown adipose tissue (BAT) in diet-induced obese mice. PLoS One 12:e0173661 (2017).

Tissue Specificity

Ubiquitous with higher expression in heart, brain and skeletal muscle.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q86YN6 Human

SwissProt: Q811R2 Rat

Synonyms

PERC antibody

peroxisome proliferative activated receptor, gamma, coactivator 1, antibody

peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody

Peroxisome proliferator-activated receptor gamma coactivator 1-beta antibody

peroxisome proliferator-activated receptor gamma, coactivator 1 beta antibody

PGC-1(beta) antibody

PGC-1-beta antibody

PGC-1-related estrogen receptor alpha coactivator antibody

PGC1 antibody

PPAR gamma coactivator-1beta antibody

Expand

Images

  • Western blot analysis of PGC1 beta on different lysates with Rabbit anti-PGC1 beta antibody (<a href="/products/ET1705-65" style="font-weight: bold;text-decoration: underline;">ET1705-65</a>) at 1/1,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: HepG2 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 113 kDa<br />Observed band size: 113 kDa<br /><br />Exposure time: 24 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-65" style="font-weight: bold;text-decoration: underline;">ET1705-65</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PGC1 beta on different lysates with Rabbit anti-PGC1 beta antibody (ET1705-65) at 1/1,000 dilution.

    Lane 1: SK-Br-3 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: HepG2 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 113 kDa
    Observed band size: 113 kDa

    Exposure time: 24 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PGC1 beta on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-65" style="font-weight: bold;text-decoration: underline;">ET1705-65</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PGC1 beta on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of PGC1 beta was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1705-65" style="font-weight: bold;text-decoration: underline;">ET1705-65</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PGC1 beta was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-65, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation