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PDLIM1 Recombinant Rabbit Monoclonal Antibody [JE55-21]

Usd: 385

Specification

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Catalog# ET7110-99

PDLIM1 Recombinant Rabbit Monoclonal Antibody [JE55-21]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

PDLIM1 Recombinant Rabbit Monoclonal Antibody [JE55-21]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human PDLIM1 aa 181-285 / 329.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 36 kDa

Positive Control

Human placenta tissue lysate, mouse lung tissue lysate, HepG2 cell lysate, rat heart tissue, HepG2, human placenta tissue, A431 cell lysate.

Conjugation

unconjugated

Clone Number

JE55-21

RRID

AB_3071057

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:1,000

  • FC

  • 1:50-1:100

Target

Function

PDLIM1, also known as CLP36, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with Actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (a kinase) and recruits Clik1 to α-actinin-1, thereby facilitating the association of Clik1 with Actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, suggesting a conserved function between species.

Background References

1. Huang Z. et. al. PDLIM1 Inhibits Tumor Metastasis Through Activating Hippo Signaling in Hepatocellular Carcinoma. Hepatology. 2019 Sep.

2. Chen HN. et. al. PDLIM1 Stabilizes the E-Cadherin/β-Catenin Complex to Prevent Epithelial-Mesenchymal Transition and Metastatic Potential of Colorectal Cancer Cells. Cancer Res. 2016 Mar

Tissue Specificity

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

Subcellular Location

Cytoskeleton, cytoplasm, Z line.

UNIPROT

SwissProt: O00151 Human

SwissProt: O70400 Mouse

SwissProt: Q3TZ17 Mouse

SwissProt: P52944 Rat

Synonyms

C terminal LIM domain protein 1 antibody

C-terminal LIM domain protein 1 antibody

Carboxy terminal LIM domain protein 1 antibody

CLIM 1 antibody

CLIM1 antibody

CLP 36 antibody

CLP36 antibody

Elfin antibody

Enigma antibody

Enigma homolog antibody

Expand

Images

  • Western blot analysis of PDLIM1 on different lysates with Rabbit anti-PDLIM1 antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>) at 1/500 dilution.<br /><br />Lane 1: Human placenta tissue lysate<br />Lane 2: Mouse lung tissue lysate<br />Lane 3: HepG2 cell lysate(10 µg/Lane)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 36 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PDLIM1 on different lysates with Rabbit anti-PDLIM1 antibody (ET7110-99) at 1/500 dilution.

    Lane 1: Human placenta tissue lysate
    Lane 2: Mouse lung tissue lysate
    Lane 3: HepG2 cell lysate(10 µg/Lane)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-99) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-PDLIM1 antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-PDLIM1 antibody (ET7110-99) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-99) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of PDLIM1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PDLIM1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-99, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PDLIM1 antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PDLIM1 antibody (ET7110-99) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of PDLIM1 on different lysates with Rabbit anti-PDLIM1 antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 cell lysate<br />Lane 2: A431 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 36 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-99" style="font-weight: bold;text-decoration: underline;">ET7110-99</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PDLIM1 on different lysates with Rabbit anti-PDLIM1 antibody (ET7110-99) at 1/1,000 dilution.

    Lane 1: A431 cell lysate
    Lane 2: A431 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-99) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"