Nudel Recombinant Rabbit Monoclonal Antibody [JG38-63]
Catalog# ET7108-73
Nudel Recombinant Rabbit Monoclonal Antibody [JG38-63]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Nudel Recombinant Rabbit Monoclonal Antibody [JG38-63]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Nudel aa 1-49 / 345.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 38 kDa
Positive Control
Mouse lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse colon tissue, rat colon tissue, human liver cancer tissue, human small intestine tissue, human uterus tissue.
Conjugation
unconjugated
Clone Number
JG38-63
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
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IHC-P
-
1:200
Target
Function
NudE-like protein (Ndel1) is expressed in the testis, brain, heart, hypothalamus, liver, lung, spleen, and stomach, specifically in the interphase centrosome and mitotic spindle. It positively regulates minus-end directed dynein. Evidence suggests that Ndel1 interacts with LIS1 to sustain the function of dynein, thereby impacting microtubule organization, nuclear translocation, and neuronal positioning. Ndel1 is phosphorylated during mitosis and seems to tether dynactin and dynein to the mother centriole for microtubule anchoring. Loss of function of Ndel1 in the developing neocortex impairs neuronal positioning and uncouples the centrosome and nucleus. Ndel1 may also impair mitochondrial transport or function, initiating a cascade of events culminating in psychiatric illness such as lissencephaly and schizophrenia.
Background References
1. Kamiya A et al. DISC1-NDEL1/NUDEL protein interaction, an essential component for neurite outgrowth, is modulated by genetic variations of DISC1. Hum Mol Genet 15:3313-3323 (2006).
2. Hayashi M A F et al. Inhibition of NUDEL (nuclear distribution element- like)-oligopeptidase activity by disrupted-in-schizophrenia 1. Proc Natl Acad Sci USA 102:3828-3833 (2005).
Sequence Similarity
Belongs to the nudE family.
Tissue Specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Post-translational Modification
Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.; Palmitoylation at Cys-273 reduces affinity for dynein.
Subcellular Location
Cytoskeleton.
Synonyms
A. nidulans antibody
DKFZp451M0318 antibody
ENDOOLIGOPEPTIDASE A antibody
EOPA antibody
MITAP 1 antibody
MITAP1 antibody
Mitosin associated protein 1 antibody
Mitosin associated protein MITAP1 antibody
Mitosin-associated protein 1 antibody
Ndel 1 antibody
ExpandImages
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Western blot analysis of Nudel on different lysates with Rabbit anti-Nudel antibody (ET7108-73) at 1/1,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-73) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Nudel antibody (ET7108-73) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Nudel antibody (ET7108-73) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-Nudel antibody (ET7108-73) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-73) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Nudel antibody (ET7108-73) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-73) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-Nudel antibody (ET7108-73) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-73) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"